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Golden Gate assembly with a bi-directional promoter (GBid): A simple, scalable method for phage display Fab library creation
Fabs offer an attractive platform for monoclonal antibody discovery/engineering, but library construction can be cumbersome. We report a simple method – Golden Gate assembly with a bi-directional promoter (GBid) – for constructing phage display Fab libraries. In GBid, the constant domains of the Fab...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7031318/ https://www.ncbi.nlm.nih.gov/pubmed/32076016 http://dx.doi.org/10.1038/s41598-020-59745-2 |