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Sucrose promotes caries progression by disrupting the microecological balance in oral biofilms: an in vitro study
Sucrose has long been regarded as the most cariogenic carbohydrate. However, why sucrose causes severer dental caries than other sugars is largely unknown. Considering that caries is a polymicrobial infection resulting from dysbiosis of oral biofilms, we hypothesized that sucrose can introduce a mic...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7031525/ https://www.ncbi.nlm.nih.gov/pubmed/32076013 http://dx.doi.org/10.1038/s41598-020-59733-6 |
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author | Du, Qian Fu, Min Zhou, Yuan Cao, Yangpei Guo, Tingwei Zhou, Zhou Li, Mingyun Peng, Xian Zheng, Xin Li, Yan Xu, Xin He, Jinzhi Zhou, Xuedong |
author_facet | Du, Qian Fu, Min Zhou, Yuan Cao, Yangpei Guo, Tingwei Zhou, Zhou Li, Mingyun Peng, Xian Zheng, Xin Li, Yan Xu, Xin He, Jinzhi Zhou, Xuedong |
author_sort | Du, Qian |
collection | PubMed |
description | Sucrose has long been regarded as the most cariogenic carbohydrate. However, why sucrose causes severer dental caries than other sugars is largely unknown. Considering that caries is a polymicrobial infection resulting from dysbiosis of oral biofilms, we hypothesized that sucrose can introduce a microbiota imbalance favoring caries to a greater degree than other sugars. To test this hypothesis, an in vitro saliva-derived multispecies biofilm model was established, and by comparing caries lesions on enamel blocks cocultured with biofilms treated with sucrose, glucose and lactose, we confirmed that this model can reproduce the in vivo finding that sucrose has the strongest cariogenic potential. In parallel, compared to a control treatment, sucrose treatment led to significant changes within the microbial structure and assembly of oral microflora, while no significant difference was detected between the lactose/glucose treatment group and the control. Specifically, sucrose supplementation disrupted the homeostasis between acid-producing and alkali-producing bacteria. Consistent with microbial dysbiosis, we observed the most significant disequilibrium between acid and alkali metabolism in sucrose-treated biofilms. Taken together, our data indicate that the cariogenicity of sugars is closely related to their ability to regulate the oral microecology. These findings advance our understanding of caries etiology from an ecological perspective. |
format | Online Article Text |
id | pubmed-7031525 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-70315252020-02-27 Sucrose promotes caries progression by disrupting the microecological balance in oral biofilms: an in vitro study Du, Qian Fu, Min Zhou, Yuan Cao, Yangpei Guo, Tingwei Zhou, Zhou Li, Mingyun Peng, Xian Zheng, Xin Li, Yan Xu, Xin He, Jinzhi Zhou, Xuedong Sci Rep Article Sucrose has long been regarded as the most cariogenic carbohydrate. However, why sucrose causes severer dental caries than other sugars is largely unknown. Considering that caries is a polymicrobial infection resulting from dysbiosis of oral biofilms, we hypothesized that sucrose can introduce a microbiota imbalance favoring caries to a greater degree than other sugars. To test this hypothesis, an in vitro saliva-derived multispecies biofilm model was established, and by comparing caries lesions on enamel blocks cocultured with biofilms treated with sucrose, glucose and lactose, we confirmed that this model can reproduce the in vivo finding that sucrose has the strongest cariogenic potential. In parallel, compared to a control treatment, sucrose treatment led to significant changes within the microbial structure and assembly of oral microflora, while no significant difference was detected between the lactose/glucose treatment group and the control. Specifically, sucrose supplementation disrupted the homeostasis between acid-producing and alkali-producing bacteria. Consistent with microbial dysbiosis, we observed the most significant disequilibrium between acid and alkali metabolism in sucrose-treated biofilms. Taken together, our data indicate that the cariogenicity of sugars is closely related to their ability to regulate the oral microecology. These findings advance our understanding of caries etiology from an ecological perspective. Nature Publishing Group UK 2020-02-19 /pmc/articles/PMC7031525/ /pubmed/32076013 http://dx.doi.org/10.1038/s41598-020-59733-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Du, Qian Fu, Min Zhou, Yuan Cao, Yangpei Guo, Tingwei Zhou, Zhou Li, Mingyun Peng, Xian Zheng, Xin Li, Yan Xu, Xin He, Jinzhi Zhou, Xuedong Sucrose promotes caries progression by disrupting the microecological balance in oral biofilms: an in vitro study |
title | Sucrose promotes caries progression by disrupting the microecological balance in oral biofilms: an in vitro study |
title_full | Sucrose promotes caries progression by disrupting the microecological balance in oral biofilms: an in vitro study |
title_fullStr | Sucrose promotes caries progression by disrupting the microecological balance in oral biofilms: an in vitro study |
title_full_unstemmed | Sucrose promotes caries progression by disrupting the microecological balance in oral biofilms: an in vitro study |
title_short | Sucrose promotes caries progression by disrupting the microecological balance in oral biofilms: an in vitro study |
title_sort | sucrose promotes caries progression by disrupting the microecological balance in oral biofilms: an in vitro study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7031525/ https://www.ncbi.nlm.nih.gov/pubmed/32076013 http://dx.doi.org/10.1038/s41598-020-59733-6 |
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