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Controlled Peptide-Mediated Vesicle Fusion Assessed by Simultaneous Dual-Colour Time-Lapsed Fluorescence Microscopy

We have employed a model system, inspired by SNARE proteins, to facilitate membrane fusion between Giant Unilamellar Vesicles (GUVs) and Large Unilamellar Vesicles (LUVs) under physiological conditions. In this system, two synthetic lipopeptide constructs comprising the coiled-coil heterodimer-formi...

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Detalles Bibliográficos
Autores principales: Mora, Nestor Lopez, Boyle, Aimee L., Kolck, Bart Jan van, Rossen, Anouk, Pokorná, Šárka, Koukalová, Alena, Šachl, Radek, Hof, Martin, Kros, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7033240/
https://www.ncbi.nlm.nih.gov/pubmed/32080270
http://dx.doi.org/10.1038/s41598-020-59926-z