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Development of a simple and sensitive HPLC-MS/MS method for determination of diazepam in human plasma and its application to a bioequivalence study
We developed a simple, sensitive, and effective ultra-performance liquid chromatography/tandem mass spectrometry (HPLC-MS/MS) method with an electrospray ionization (ESI) interface in multiple reaction monitoring (MRM) and positive ion modes to determine diazepam concentrations in human plasma using...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society for Clinical Pharmacology and Therapeutics
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7033404/ https://www.ncbi.nlm.nih.gov/pubmed/32095471 http://dx.doi.org/10.12793/tcp.2017.25.4.173 |
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author | Kim, Do-Hyung Cho, Ji-Yoon Chae, Soo-In Kang, Bo-Kyung An, Tae-Gil Shim, Wang-Seob Noh, Young Su Hwang, Se Jung Chung, Eun Kyoung Lee, Kyung-Tae |
author_facet | Kim, Do-Hyung Cho, Ji-Yoon Chae, Soo-In Kang, Bo-Kyung An, Tae-Gil Shim, Wang-Seob Noh, Young Su Hwang, Se Jung Chung, Eun Kyoung Lee, Kyung-Tae |
author_sort | Kim, Do-Hyung |
collection | PubMed |
description | We developed a simple, sensitive, and effective ultra-performance liquid chromatography/tandem mass spectrometry (HPLC-MS/MS) method with an electrospray ionization (ESI) interface in multiple reaction monitoring (MRM) and positive ion modes to determine diazepam concentrations in human plasma using voriconazole as an internal standard (IS). Diazepam and IS were detected at transition 285.2→193.1 and 350.2→127.1, respectively. After liquid-liquid extraction (LLE) using 1.2 ml of ethyl acetate:n-hexane (80:20, v/v), diazepam and IS were eluted on a Phenomenex Cadenza CD-C18 column (150 × 3.0 mm, 3 µm) with an isocratic mobile phase (10 mM ammonium acetate in water:methanol [5:95, v/v]) at a flow rate of 0.4 mL/min. The peak retention time was 2.32 min for diazepam and 2.01 min for IS, respectively. The lower limit of quantitation (LLOQ) was 0.5 ng/mL (S/N > 10) using 50 µL of plasma, and no interferences were observed in chromatograms. Our analytical method was fully validated and successfully applied to a bioequivalence study of two formulations of diazepam in healthy Korean volunteers. |
format | Online Article Text |
id | pubmed-7033404 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Korean Society for Clinical Pharmacology and Therapeutics |
record_format | MEDLINE/PubMed |
spelling | pubmed-70334042020-02-24 Development of a simple and sensitive HPLC-MS/MS method for determination of diazepam in human plasma and its application to a bioequivalence study Kim, Do-Hyung Cho, Ji-Yoon Chae, Soo-In Kang, Bo-Kyung An, Tae-Gil Shim, Wang-Seob Noh, Young Su Hwang, Se Jung Chung, Eun Kyoung Lee, Kyung-Tae Transl Clin Pharmacol Original Article We developed a simple, sensitive, and effective ultra-performance liquid chromatography/tandem mass spectrometry (HPLC-MS/MS) method with an electrospray ionization (ESI) interface in multiple reaction monitoring (MRM) and positive ion modes to determine diazepam concentrations in human plasma using voriconazole as an internal standard (IS). Diazepam and IS were detected at transition 285.2→193.1 and 350.2→127.1, respectively. After liquid-liquid extraction (LLE) using 1.2 ml of ethyl acetate:n-hexane (80:20, v/v), diazepam and IS were eluted on a Phenomenex Cadenza CD-C18 column (150 × 3.0 mm, 3 µm) with an isocratic mobile phase (10 mM ammonium acetate in water:methanol [5:95, v/v]) at a flow rate of 0.4 mL/min. The peak retention time was 2.32 min for diazepam and 2.01 min for IS, respectively. The lower limit of quantitation (LLOQ) was 0.5 ng/mL (S/N > 10) using 50 µL of plasma, and no interferences were observed in chromatograms. Our analytical method was fully validated and successfully applied to a bioequivalence study of two formulations of diazepam in healthy Korean volunteers. Korean Society for Clinical Pharmacology and Therapeutics 2017-12 2017-12-20 /pmc/articles/PMC7033404/ /pubmed/32095471 http://dx.doi.org/10.12793/tcp.2017.25.4.173 Text en Copyright © 2017 Translational and Clinical Pharmacology http://creativecommons.org/licenses/by-nc/3.0/ It is identical to the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/). |
spellingShingle | Original Article Kim, Do-Hyung Cho, Ji-Yoon Chae, Soo-In Kang, Bo-Kyung An, Tae-Gil Shim, Wang-Seob Noh, Young Su Hwang, Se Jung Chung, Eun Kyoung Lee, Kyung-Tae Development of a simple and sensitive HPLC-MS/MS method for determination of diazepam in human plasma and its application to a bioequivalence study |
title | Development of a simple and sensitive HPLC-MS/MS method for determination of diazepam in human plasma and its application to a bioequivalence study |
title_full | Development of a simple and sensitive HPLC-MS/MS method for determination of diazepam in human plasma and its application to a bioequivalence study |
title_fullStr | Development of a simple and sensitive HPLC-MS/MS method for determination of diazepam in human plasma and its application to a bioequivalence study |
title_full_unstemmed | Development of a simple and sensitive HPLC-MS/MS method for determination of diazepam in human plasma and its application to a bioequivalence study |
title_short | Development of a simple and sensitive HPLC-MS/MS method for determination of diazepam in human plasma and its application to a bioequivalence study |
title_sort | development of a simple and sensitive hplc-ms/ms method for determination of diazepam in human plasma and its application to a bioequivalence study |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7033404/ https://www.ncbi.nlm.nih.gov/pubmed/32095471 http://dx.doi.org/10.12793/tcp.2017.25.4.173 |
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