A Single Tube Overlap Extension PCR Method for Splicing of Multiple DNA Fragments

BACKGROUND: Despite the ease of conventional splicing by overlap-extension (SOEing) PCR technique in theory, when splicing more than two fragments, and especially if one of the complementary sequences is A-T rich, the attachment of the fragments would be challenging. A new rapid and highly efficient...

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Detalles Bibliográficos
Autores principales: Zarghampoor, Farzaneh, Behzad-Behbahani, Abbas, Azarpira, Negar, Khatami, Saeed Reza, Fanian, Maryam, Hossein Aghdaie, Mahdokht, Rafiei Dehbidi, Gholamreza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Avicenna Research Institute 2020
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035467/
https://www.ncbi.nlm.nih.gov/pubmed/32153737

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035467/
https://www.ncbi.nlm.nih.gov/pubmed/32153737

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