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Modulation of Allicin-Free Garlic on Gut Microbiome
The allicin diallyldisulfid-S-oxide, a major garlic organosulfur compound (OSC) in crushed garlic (Allium sativum L.), possesses antibacterial effects, and influences gut bacteria. In this study, we made allicin-free garlic (AFG) extract and investigated its effects on gut microbiome. C57BL/6N male...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036987/ https://www.ncbi.nlm.nih.gov/pubmed/32033507 http://dx.doi.org/10.3390/molecules25030682 |
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author | Chen, Keyu Nakasone, Yasushi Xie, Kun Sakao, Kozue Hou, De-Xing |
author_facet | Chen, Keyu Nakasone, Yasushi Xie, Kun Sakao, Kozue Hou, De-Xing |
author_sort | Chen, Keyu |
collection | PubMed |
description | The allicin diallyldisulfid-S-oxide, a major garlic organosulfur compound (OSC) in crushed garlic (Allium sativum L.), possesses antibacterial effects, and influences gut bacteria. In this study, we made allicin-free garlic (AFG) extract and investigated its effects on gut microbiome. C57BL/6N male mice were randomly divided into 6 groups and fed normal diet (ND) and high-fat diet (HFD) supplemented with or without AFG in concentrations of 1% and 5% for 11 weeks. The genomic DNAs of feces were used to identify the gut microbiome by sequencing 16S rRNA genes. The results revealed that the ratio of p-Firmicutes to p-Bacteroidetes increased by aging and HFD was reduced by AFG. In particular, the f-Lachnospiraceae, g-Akkermansia, and g-Lactobacillus decreased by aging and HFD was enhanced by AFG. The g-Dorea increased by aging and HFD decreased by AFG. In addition, the ratio of glutamic-pyruvic transaminase to glutamic-oxaloacetic transaminase (GPT/GOT) in serum was significantly increased in the HFD group and decreased by AFG. In summary, our data demonstrated that dietary intervention with AFG is a potential way to balance the gut microbiome disturbed by a high-fat diet. |
format | Online Article Text |
id | pubmed-7036987 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70369872020-03-11 Modulation of Allicin-Free Garlic on Gut Microbiome Chen, Keyu Nakasone, Yasushi Xie, Kun Sakao, Kozue Hou, De-Xing Molecules Article The allicin diallyldisulfid-S-oxide, a major garlic organosulfur compound (OSC) in crushed garlic (Allium sativum L.), possesses antibacterial effects, and influences gut bacteria. In this study, we made allicin-free garlic (AFG) extract and investigated its effects on gut microbiome. C57BL/6N male mice were randomly divided into 6 groups and fed normal diet (ND) and high-fat diet (HFD) supplemented with or without AFG in concentrations of 1% and 5% for 11 weeks. The genomic DNAs of feces were used to identify the gut microbiome by sequencing 16S rRNA genes. The results revealed that the ratio of p-Firmicutes to p-Bacteroidetes increased by aging and HFD was reduced by AFG. In particular, the f-Lachnospiraceae, g-Akkermansia, and g-Lactobacillus decreased by aging and HFD was enhanced by AFG. The g-Dorea increased by aging and HFD decreased by AFG. In addition, the ratio of glutamic-pyruvic transaminase to glutamic-oxaloacetic transaminase (GPT/GOT) in serum was significantly increased in the HFD group and decreased by AFG. In summary, our data demonstrated that dietary intervention with AFG is a potential way to balance the gut microbiome disturbed by a high-fat diet. MDPI 2020-02-05 /pmc/articles/PMC7036987/ /pubmed/32033507 http://dx.doi.org/10.3390/molecules25030682 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chen, Keyu Nakasone, Yasushi Xie, Kun Sakao, Kozue Hou, De-Xing Modulation of Allicin-Free Garlic on Gut Microbiome |
title | Modulation of Allicin-Free Garlic on Gut Microbiome |
title_full | Modulation of Allicin-Free Garlic on Gut Microbiome |
title_fullStr | Modulation of Allicin-Free Garlic on Gut Microbiome |
title_full_unstemmed | Modulation of Allicin-Free Garlic on Gut Microbiome |
title_short | Modulation of Allicin-Free Garlic on Gut Microbiome |
title_sort | modulation of allicin-free garlic on gut microbiome |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7036987/ https://www.ncbi.nlm.nih.gov/pubmed/32033507 http://dx.doi.org/10.3390/molecules25030682 |
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