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A Comparison of Osteoblast and Osteoclast In Vitro Co-Culture Models and Their Translation for Preclinical Drug Testing Applications

As the population of western societies on average ages, the number of people affected by bone remodeling-associated diseases such as osteoporosis continues to increase. The development of new therapeutics is hampered by the high failure rates of drug candidates during clinical testing, which is in p...

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Autores principales: Sieberath, Alexander, Della Bella, Elena, Ferreira, Ana Marina, Gentile, Piergiorgio, Eglin, David, Dalgarno, Kenny
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037207/
https://www.ncbi.nlm.nih.gov/pubmed/32019244
http://dx.doi.org/10.3390/ijms21030912
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author Sieberath, Alexander
Della Bella, Elena
Ferreira, Ana Marina
Gentile, Piergiorgio
Eglin, David
Dalgarno, Kenny
author_facet Sieberath, Alexander
Della Bella, Elena
Ferreira, Ana Marina
Gentile, Piergiorgio
Eglin, David
Dalgarno, Kenny
author_sort Sieberath, Alexander
collection PubMed
description As the population of western societies on average ages, the number of people affected by bone remodeling-associated diseases such as osteoporosis continues to increase. The development of new therapeutics is hampered by the high failure rates of drug candidates during clinical testing, which is in part due to the poor predictive character of animal models during preclinical drug testing. Co-culture models of osteoblasts and osteoclasts offer an alternative to animal testing and are considered to have the potential to improve drug development processes in the future. However, a robust, scalable, and reproducible 3D model combining osteoblasts and osteoclasts for preclinical drug testing purposes has not been developed to date. Here we review various types of osteoblast–osteoclast co-culture models and outline the remaining obstacles that must be overcome for their successful translation.
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spelling pubmed-70372072020-03-11 A Comparison of Osteoblast and Osteoclast In Vitro Co-Culture Models and Their Translation for Preclinical Drug Testing Applications Sieberath, Alexander Della Bella, Elena Ferreira, Ana Marina Gentile, Piergiorgio Eglin, David Dalgarno, Kenny Int J Mol Sci Review As the population of western societies on average ages, the number of people affected by bone remodeling-associated diseases such as osteoporosis continues to increase. The development of new therapeutics is hampered by the high failure rates of drug candidates during clinical testing, which is in part due to the poor predictive character of animal models during preclinical drug testing. Co-culture models of osteoblasts and osteoclasts offer an alternative to animal testing and are considered to have the potential to improve drug development processes in the future. However, a robust, scalable, and reproducible 3D model combining osteoblasts and osteoclasts for preclinical drug testing purposes has not been developed to date. Here we review various types of osteoblast–osteoclast co-culture models and outline the remaining obstacles that must be overcome for their successful translation. MDPI 2020-01-30 /pmc/articles/PMC7037207/ /pubmed/32019244 http://dx.doi.org/10.3390/ijms21030912 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Sieberath, Alexander
Della Bella, Elena
Ferreira, Ana Marina
Gentile, Piergiorgio
Eglin, David
Dalgarno, Kenny
A Comparison of Osteoblast and Osteoclast In Vitro Co-Culture Models and Their Translation for Preclinical Drug Testing Applications
title A Comparison of Osteoblast and Osteoclast In Vitro Co-Culture Models and Their Translation for Preclinical Drug Testing Applications
title_full A Comparison of Osteoblast and Osteoclast In Vitro Co-Culture Models and Their Translation for Preclinical Drug Testing Applications
title_fullStr A Comparison of Osteoblast and Osteoclast In Vitro Co-Culture Models and Their Translation for Preclinical Drug Testing Applications
title_full_unstemmed A Comparison of Osteoblast and Osteoclast In Vitro Co-Culture Models and Their Translation for Preclinical Drug Testing Applications
title_short A Comparison of Osteoblast and Osteoclast In Vitro Co-Culture Models and Their Translation for Preclinical Drug Testing Applications
title_sort comparison of osteoblast and osteoclast in vitro co-culture models and their translation for preclinical drug testing applications
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037207/
https://www.ncbi.nlm.nih.gov/pubmed/32019244
http://dx.doi.org/10.3390/ijms21030912
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