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Effect of Pepper-Containing Diets on the Diversity and Composition of Gut Microbiome of Drosophila melanogaster

One of the greatest impacts on the gastrointestinal microbiome is diet because the host and microbiome share the same food source. In addition, the effect of diet can diverge depending on the host genotype. Diets supplemented with phytochemicals found in peppers might cause shifts in the microbiome....

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Autores principales: Garcia-Lozano, Marleny, Haynes, Joshua, Lopez-Ortiz, Carlos, Natarajan, Purushothaman, Peña-Garcia, Yadira, Nimmakayala, Padma, Stommel, John, Alaparthi, Suresh B., Sirbu, Cristian, Balagurusamy, Nagamani, Reddy, Umesh K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7038135/
https://www.ncbi.nlm.nih.gov/pubmed/32023882
http://dx.doi.org/10.3390/ijms21030945
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author Garcia-Lozano, Marleny
Haynes, Joshua
Lopez-Ortiz, Carlos
Natarajan, Purushothaman
Peña-Garcia, Yadira
Nimmakayala, Padma
Stommel, John
Alaparthi, Suresh B.
Sirbu, Cristian
Balagurusamy, Nagamani
Reddy, Umesh K.
author_facet Garcia-Lozano, Marleny
Haynes, Joshua
Lopez-Ortiz, Carlos
Natarajan, Purushothaman
Peña-Garcia, Yadira
Nimmakayala, Padma
Stommel, John
Alaparthi, Suresh B.
Sirbu, Cristian
Balagurusamy, Nagamani
Reddy, Umesh K.
author_sort Garcia-Lozano, Marleny
collection PubMed
description One of the greatest impacts on the gastrointestinal microbiome is diet because the host and microbiome share the same food source. In addition, the effect of diet can diverge depending on the host genotype. Diets supplemented with phytochemicals found in peppers might cause shifts in the microbiome. Thus, understanding how these interactions occur can reveal potential health implications associated with such changes. This study aims to explore the gut microbiome of different Drosophila genetic backgrounds and the effects of dietary pepper treatments on its composition and structure. We analyzed the gut microbiomes of three Drosophila melanogaster genetic backgrounds (Canton-S, Oregon-RC, and Berlin-K) reared on control and pepper-containing diets (bell, serrano, and habanero peppers). Results of 16S rRNA gene sequencing revealed that the variability of Drosophila gut microbiome can be driven mainly by genetic factors. When the abundance of these communities is considered, pepper-containing diets also appear to have an effect. The most relevant change in microbial composition was the increment of Lactobacillaceae and Acetobacteraceae abundance in the pepper-containing diets in comparison with the controls in Oregon-RC and Berlin-K. Regression analysis demonstrated that this enhancement was associated with the content of phenolic compounds and carotenoids of the peppers utilized in this study; specifically, to the concentration of β-carotene, β-cryptoxanthin, myricetin, quercetin, and apigenin.
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spelling pubmed-70381352020-03-10 Effect of Pepper-Containing Diets on the Diversity and Composition of Gut Microbiome of Drosophila melanogaster Garcia-Lozano, Marleny Haynes, Joshua Lopez-Ortiz, Carlos Natarajan, Purushothaman Peña-Garcia, Yadira Nimmakayala, Padma Stommel, John Alaparthi, Suresh B. Sirbu, Cristian Balagurusamy, Nagamani Reddy, Umesh K. Int J Mol Sci Article One of the greatest impacts on the gastrointestinal microbiome is diet because the host and microbiome share the same food source. In addition, the effect of diet can diverge depending on the host genotype. Diets supplemented with phytochemicals found in peppers might cause shifts in the microbiome. Thus, understanding how these interactions occur can reveal potential health implications associated with such changes. This study aims to explore the gut microbiome of different Drosophila genetic backgrounds and the effects of dietary pepper treatments on its composition and structure. We analyzed the gut microbiomes of three Drosophila melanogaster genetic backgrounds (Canton-S, Oregon-RC, and Berlin-K) reared on control and pepper-containing diets (bell, serrano, and habanero peppers). Results of 16S rRNA gene sequencing revealed that the variability of Drosophila gut microbiome can be driven mainly by genetic factors. When the abundance of these communities is considered, pepper-containing diets also appear to have an effect. The most relevant change in microbial composition was the increment of Lactobacillaceae and Acetobacteraceae abundance in the pepper-containing diets in comparison with the controls in Oregon-RC and Berlin-K. Regression analysis demonstrated that this enhancement was associated with the content of phenolic compounds and carotenoids of the peppers utilized in this study; specifically, to the concentration of β-carotene, β-cryptoxanthin, myricetin, quercetin, and apigenin. MDPI 2020-01-31 /pmc/articles/PMC7038135/ /pubmed/32023882 http://dx.doi.org/10.3390/ijms21030945 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Garcia-Lozano, Marleny
Haynes, Joshua
Lopez-Ortiz, Carlos
Natarajan, Purushothaman
Peña-Garcia, Yadira
Nimmakayala, Padma
Stommel, John
Alaparthi, Suresh B.
Sirbu, Cristian
Balagurusamy, Nagamani
Reddy, Umesh K.
Effect of Pepper-Containing Diets on the Diversity and Composition of Gut Microbiome of Drosophila melanogaster
title Effect of Pepper-Containing Diets on the Diversity and Composition of Gut Microbiome of Drosophila melanogaster
title_full Effect of Pepper-Containing Diets on the Diversity and Composition of Gut Microbiome of Drosophila melanogaster
title_fullStr Effect of Pepper-Containing Diets on the Diversity and Composition of Gut Microbiome of Drosophila melanogaster
title_full_unstemmed Effect of Pepper-Containing Diets on the Diversity and Composition of Gut Microbiome of Drosophila melanogaster
title_short Effect of Pepper-Containing Diets on the Diversity and Composition of Gut Microbiome of Drosophila melanogaster
title_sort effect of pepper-containing diets on the diversity and composition of gut microbiome of drosophila melanogaster
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7038135/
https://www.ncbi.nlm.nih.gov/pubmed/32023882
http://dx.doi.org/10.3390/ijms21030945
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