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Akt inhibitor deguelin aggravates inflammation and fibrosis in myocarditis

OBJECTIVE(S): Myocarditis is characterized by inflammatory cell infiltration in myocardial stroma. Attenuation of tumor necrosis factor (TNF)-α and interleukin (IL)-1β is a reliable mark for improving the prognosis. Protein kinase B (Akt) plays an important role in the development and progression of...

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Autores principales: Li, Shanshan, Wang, Yue, Zhao, Chunming, Zhang, Meixiang, Wang, Wei, Yu, Xiaowei, Huang, Jiao, Wang, Zhao, Zhu, Bo, Yin, Chengqian, Cai, Hongxing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7038425/
https://www.ncbi.nlm.nih.gov/pubmed/32128091
http://dx.doi.org/10.22038/ijbms.2019.35518.8473
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author Li, Shanshan
Wang, Yue
Zhao, Chunming
Zhang, Meixiang
Wang, Wei
Yu, Xiaowei
Huang, Jiao
Wang, Zhao
Zhu, Bo
Yin, Chengqian
Cai, Hongxing
author_facet Li, Shanshan
Wang, Yue
Zhao, Chunming
Zhang, Meixiang
Wang, Wei
Yu, Xiaowei
Huang, Jiao
Wang, Zhao
Zhu, Bo
Yin, Chengqian
Cai, Hongxing
author_sort Li, Shanshan
collection PubMed
description OBJECTIVE(S): Myocarditis is characterized by inflammatory cell infiltration in myocardial stroma. Attenuation of tumor necrosis factor (TNF)-α and interleukin (IL)-1β is a reliable mark for improving the prognosis. Protein kinase B (Akt) plays an important role in the development and progression of myocarditis. The specific role of the natural inhibitor of Akt, Deguelin, on myocarditis has not been reported. In this study, we used deguelin to investigate the effects of natural Akt inhibitor on myocarditis in experimental autoimmune myocarditis (EAM) rats. MATERIALS AND METHODS: EAM rat models were made by using Lewis rats and Deguelin was injected intraperitoneally on day 3, 6, 9, 12 and 15 after successful modeling. On day 18, rats were sacrificed and the heart weight (HW)/ body weight (BW) ratio were measured. The pathological changes, pathological scores and fibrosis area were evaluated after H.&E. and Masson’s trichrome staining. The mRNA levels of TNF-α and IL-1β were measured by RT-qPCR, while the protein expressions of TNF-α and IL-1β were detected by immunohistochemical staining and Western bolt. The protein expressions of Akt, Akt1, phosphorylated (p-) Akt and nuclear factor (NF)-κB were detected by Western bolt. RESULTS: We found that the TNF-α and IL-1β levels, inflammatory scores and fibrosis areas were markedly increased after 18 days deguelin administration. CONCLUSION: Akt inhibition with deguelin may aggravate myocarditis of EAM rats.
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spelling pubmed-70384252020-03-03 Akt inhibitor deguelin aggravates inflammation and fibrosis in myocarditis Li, Shanshan Wang, Yue Zhao, Chunming Zhang, Meixiang Wang, Wei Yu, Xiaowei Huang, Jiao Wang, Zhao Zhu, Bo Yin, Chengqian Cai, Hongxing Iran J Basic Med Sci Research Article OBJECTIVE(S): Myocarditis is characterized by inflammatory cell infiltration in myocardial stroma. Attenuation of tumor necrosis factor (TNF)-α and interleukin (IL)-1β is a reliable mark for improving the prognosis. Protein kinase B (Akt) plays an important role in the development and progression of myocarditis. The specific role of the natural inhibitor of Akt, Deguelin, on myocarditis has not been reported. In this study, we used deguelin to investigate the effects of natural Akt inhibitor on myocarditis in experimental autoimmune myocarditis (EAM) rats. MATERIALS AND METHODS: EAM rat models were made by using Lewis rats and Deguelin was injected intraperitoneally on day 3, 6, 9, 12 and 15 after successful modeling. On day 18, rats were sacrificed and the heart weight (HW)/ body weight (BW) ratio were measured. The pathological changes, pathological scores and fibrosis area were evaluated after H.&E. and Masson’s trichrome staining. The mRNA levels of TNF-α and IL-1β were measured by RT-qPCR, while the protein expressions of TNF-α and IL-1β were detected by immunohistochemical staining and Western bolt. The protein expressions of Akt, Akt1, phosphorylated (p-) Akt and nuclear factor (NF)-κB were detected by Western bolt. RESULTS: We found that the TNF-α and IL-1β levels, inflammatory scores and fibrosis areas were markedly increased after 18 days deguelin administration. CONCLUSION: Akt inhibition with deguelin may aggravate myocarditis of EAM rats. Mashhad University of Medical Sciences 2019-11 /pmc/articles/PMC7038425/ /pubmed/32128091 http://dx.doi.org/10.22038/ijbms.2019.35518.8473 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Shanshan
Wang, Yue
Zhao, Chunming
Zhang, Meixiang
Wang, Wei
Yu, Xiaowei
Huang, Jiao
Wang, Zhao
Zhu, Bo
Yin, Chengqian
Cai, Hongxing
Akt inhibitor deguelin aggravates inflammation and fibrosis in myocarditis
title Akt inhibitor deguelin aggravates inflammation and fibrosis in myocarditis
title_full Akt inhibitor deguelin aggravates inflammation and fibrosis in myocarditis
title_fullStr Akt inhibitor deguelin aggravates inflammation and fibrosis in myocarditis
title_full_unstemmed Akt inhibitor deguelin aggravates inflammation and fibrosis in myocarditis
title_short Akt inhibitor deguelin aggravates inflammation and fibrosis in myocarditis
title_sort akt inhibitor deguelin aggravates inflammation and fibrosis in myocarditis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7038425/
https://www.ncbi.nlm.nih.gov/pubmed/32128091
http://dx.doi.org/10.22038/ijbms.2019.35518.8473
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