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Reassembling green fluorescent protein for in vitro evaluation of trans-translation

In order to discover new antibiotics with improved activity and selectivity, we created a reliable in vitro reporter system to detect trans-translation activity, the main mechanism for recycling ribosomes stalled on problematic messenger RNA (mRNA) in bacteria. This system is based on an engineered...

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Detalles Bibliográficos
Autores principales: Guyomar, Charlotte, Thépaut, Marion, Nonin-Lecomte, Sylvie, Méreau, Agnès, Goude, Renan, Gillet, Reynald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7038980/
https://www.ncbi.nlm.nih.gov/pubmed/31919515
http://dx.doi.org/10.1093/nar/gkz1204