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The liquid state of FG-nucleoporins mimics permeability barrier properties of nuclear pore complexes

Nuclear pore complexes (NPCs) regulate all cargo traffic across the nuclear envelope. The transport conduit of NPCs is highly enriched in disordered phenylalanine/glycine-rich nucleoporins (FG-Nups), which form a permeability barrier of still elusive and highly debated molecular structure. Here we p...

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Autores principales: Celetti, Giorgia, Paci, Giulia, Caria, Joana, VanDelinder, Virginia, Bachand, George, Lemke, Edward A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039189/
https://www.ncbi.nlm.nih.gov/pubmed/31723007
http://dx.doi.org/10.1083/jcb.201907157
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author Celetti, Giorgia
Paci, Giulia
Caria, Joana
VanDelinder, Virginia
Bachand, George
Lemke, Edward A.
author_facet Celetti, Giorgia
Paci, Giulia
Caria, Joana
VanDelinder, Virginia
Bachand, George
Lemke, Edward A.
author_sort Celetti, Giorgia
collection PubMed
description Nuclear pore complexes (NPCs) regulate all cargo traffic across the nuclear envelope. The transport conduit of NPCs is highly enriched in disordered phenylalanine/glycine-rich nucleoporins (FG-Nups), which form a permeability barrier of still elusive and highly debated molecular structure. Here we present a microfluidic device that triggered liquid-to-liquid phase separation of FG-Nups, which yielded droplets that showed typical properties of a liquid state. On the microfluidic chip, droplets were perfused with different transport-competent or -incompetent cargo complexes, and then the permeability barrier properties of the droplets were optically interrogated. We show that the liquid state mimics permeability barrier properties of the physiological nuclear transport pathway in intact NPCs in cells: that is, inert cargoes ranging from small proteins to large capsids were excluded from liquid FG-Nup droplets, but functional import complexes underwent facilitated import into droplets. Collectively, these data provide an experimental model of how NPCs can facilitate fast passage of cargoes across an order of magnitude in cargo size.
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spelling pubmed-70391892020-07-06 The liquid state of FG-nucleoporins mimics permeability barrier properties of nuclear pore complexes Celetti, Giorgia Paci, Giulia Caria, Joana VanDelinder, Virginia Bachand, George Lemke, Edward A. J Cell Biol Research Articles Nuclear pore complexes (NPCs) regulate all cargo traffic across the nuclear envelope. The transport conduit of NPCs is highly enriched in disordered phenylalanine/glycine-rich nucleoporins (FG-Nups), which form a permeability barrier of still elusive and highly debated molecular structure. Here we present a microfluidic device that triggered liquid-to-liquid phase separation of FG-Nups, which yielded droplets that showed typical properties of a liquid state. On the microfluidic chip, droplets were perfused with different transport-competent or -incompetent cargo complexes, and then the permeability barrier properties of the droplets were optically interrogated. We show that the liquid state mimics permeability barrier properties of the physiological nuclear transport pathway in intact NPCs in cells: that is, inert cargoes ranging from small proteins to large capsids were excluded from liquid FG-Nup droplets, but functional import complexes underwent facilitated import into droplets. Collectively, these data provide an experimental model of how NPCs can facilitate fast passage of cargoes across an order of magnitude in cargo size. Rockefeller University Press 2019-11-13 /pmc/articles/PMC7039189/ /pubmed/31723007 http://dx.doi.org/10.1083/jcb.201907157 Text en © 2019 Celetti et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Celetti, Giorgia
Paci, Giulia
Caria, Joana
VanDelinder, Virginia
Bachand, George
Lemke, Edward A.
The liquid state of FG-nucleoporins mimics permeability barrier properties of nuclear pore complexes
title The liquid state of FG-nucleoporins mimics permeability barrier properties of nuclear pore complexes
title_full The liquid state of FG-nucleoporins mimics permeability barrier properties of nuclear pore complexes
title_fullStr The liquid state of FG-nucleoporins mimics permeability barrier properties of nuclear pore complexes
title_full_unstemmed The liquid state of FG-nucleoporins mimics permeability barrier properties of nuclear pore complexes
title_short The liquid state of FG-nucleoporins mimics permeability barrier properties of nuclear pore complexes
title_sort liquid state of fg-nucleoporins mimics permeability barrier properties of nuclear pore complexes
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039189/
https://www.ncbi.nlm.nih.gov/pubmed/31723007
http://dx.doi.org/10.1083/jcb.201907157
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