Flow fluorometry quantification of anion channel VRAC subunit LRRC8A at the membrane of living U937 cells

Assessing the expression of channels on the cell membrane is a necessary step in studying the functioning of ion channels in living cells. We explore, first, if endogenous VRAC can be assayed using flow cytometry and a commercially available antibody against an extracellular loop of the LRRC8A, also...

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Detalles Bibliográficos
Autores principales: Yurinskaya, Valentina, Aksenov, Nikolay, Moshkov, Alexey, Goryachaya, Tatyana, Shemery, Ashley, Vereninov, Alexey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039630/
https://www.ncbi.nlm.nih.gov/pubmed/32075501
http://dx.doi.org/10.1080/19336950.2020.1730535

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039630/
https://www.ncbi.nlm.nih.gov/pubmed/32075501
http://dx.doi.org/10.1080/19336950.2020.1730535

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