Cargando…

Flow fluorometry quantification of anion channel VRAC subunit LRRC8A at the membrane of living U937 cells

Assessing the expression of channels on the cell membrane is a necessary step in studying the functioning of ion channels in living cells. We explore, first, if endogenous VRAC can be assayed using flow cytometry and a commercially available antibody against an extracellular loop of the LRRC8A, also...

Descripción completa

Detalles Bibliográficos
Autores principales:	Yurinskaya, Valentina, Aksenov, Nikolay, Moshkov, Alexey, Goryachaya, Tatyana, Shemery, Ashley, Vereninov, Alexey
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Taylor & Francis 2020
Materias:	Brief Report
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039630/ https://www.ncbi.nlm.nih.gov/pubmed/32075501 http://dx.doi.org/10.1080/19336950.2020.1730535

Ejemplares similares

Unidirectional fluxes of monovalent ions in human erythrocytes compared with lymphoid U937 cells: Transient processes after stopping the sodium pump and in response to osmotic challenge
por: Yurinskaya, Valentina E., et al.
Publicado: (2023)

Balance of Na(+), K(+), and Cl(–) Unidirectional Fluxes in Normal and Apoptotic U937 Cells Computed With All Main Types of Cotransporters
por: Yurinskaya, Valentina E., et al.
Publicado: (2020)

Structure-function relationships of the LRRC8 subunits and subdomains of the volume-regulated anion channel (VRAC)
por: Ghouli, Manolia R., et al.
Publicado: (2022)

Unidirectional Flux Balance of Monovalent Ions in Cells with Na/Na and Li/Na Exchange: Experimental and Computational Studies on Lymphoid U937 Cells
por: Vereninov, Igor A., et al.
Publicado: (2016)

Cation-Chloride Cotransporters, Na/K Pump, and Channels in Cell Water and Ion Regulation: In silico and Experimental Studies of the U937 Cells Under Stopping the Pump and During Regulatory Volume Decrease
por: Yurinskaya, Valentina E., et al.
Publicado: (2021)

Cation-Chloride Cotransporters, Na/K Pump, and Channels in Cell Water/Ionic Balance Regulation Under Hyperosmolar Conditions: In Silico and Experimental Studies of Opposite RVI and AVD Responses of U937 Cells to Hyperosmolar Media
por: Yurinskaya, Valentina E., et al.
Publicado: (2022)

LRRC8/VRAC anion channels are required for late stages of spermatid development in mice
por: Lück, Jennifer C., et al.
Publicado: (2018)

The Volume-Regulated Anion Channel LRRC8/VRAC Is Dispensable for Cell Proliferation and Migration
por: Liu, Tianbao, et al.
Publicado: (2019)

LRRC8/VRAC anion channels enhance β-cell glucose sensing and insulin secretion
por: Stuhlmann, Till, et al.
Publicado: (2018)

LRRC8 N termini influence pore properties and gating of volume-regulated anion channels (VRACs)
por: Zhou, Pingzheng, et al.
Publicado: (2018)

Absolute Protein Amounts and Relative Abundance of Volume-regulated Anion Channel (VRAC) LRRC8 Subunits in Cells and Tissues Revealed by Quantitative Immunoblotting
por: Pervaiz, Sumaira, et al.
Publicado: (2019)

Expression of LRRC8/VRAC Currents in Xenopus Oocytes: Advantages and Caveats
por: Gaitán-Peñas, Héctor, et al.
Publicado: (2018)

Intracellular K(+) and water content in human blood lymphocytes during transition from quiescence to proliferation
por: Marakhova, Irina, et al.
Publicado: (2019)

Comparative Effects of Chloride Channel Inhibitors on LRRC8/VRAC-Mediated Chloride Conductance
por: Friard, Jonas, et al.
Publicado: (2017)

Commentary: How Cells Can Control Their Size by Pumping Ions
por: Vereninov, Igor A., et al.
Publicado: (2017)

A Tool for Computation of Changes in Na(+), K(+), Cl(−) Channels and Transporters Due to Apoptosis by Data on Cell Ion and Water Content Alteration
por: Yurinskaya, Valentina E., et al.
Publicado: (2019)

Ca(2+) Dependence of Volume-Regulated VRAC/LRRC8 and TMEM16A Cl(–) Channels
por: Centeio, Raquel, et al.
Publicado: (2020)

LRRC8/VRAC channels exhibit a noncanonical permeability to glutathione, which modulates epithelial-to-mesenchymal transition (EMT)
por: Friard, Jonas, et al.
Publicado: (2019)

Tweety-homolog (Ttyh) Family Encodes the Pore-forming Subunits of the Swelling-dependent Volume-regulated Anion Channel (VRAC(swell)) in the Brain
por: Han, Young-Eun, et al.
Publicado: (2019)

A SWELL time to develop the molecular pharmacology of the volume-regulated anion channel (VRAC)
por: Figueroa, Eric E., et al.
Publicado: (2022)

Proteasome (Prosome) Subunit Variations during the Differentiation of Myeloid U937 Cells
por: Henry, Laurent, et al.
Publicado: (1997)

Trends in volume-regulated anion channel (VRAC) research: visualization and bibliometric analysis from 2014 to 2022
por: Liu, Tianbao, et al.
Publicado: (2023)

Late adolescence mortality in mice with brain-specific deletion of the volume-regulated anion channel subunit LRRC8A
por: Wilson, Corinne S., et al.
Publicado: (2021)

The Magical Combination of Polymer Science and Fluorometry
por: Uchiyama, Seiichi
Publicado: (2020)

Structural insights into anion selectivity and activation mechanism of LRRC8 volume-regulated anion channels
por: Liu, Heng, et al.
Publicado: (2023)

Loss of the Volume-regulated Anion Channel Components LRRC8A and LRRC8D Limits Platinum Drug Efficacy
por: Widmer, Carmen A., et al.
Publicado: (2022)

The LRRC8-mediated volume-regulated anion channel is altered in glaucoma
por: Gasull, Xavier, et al.
Publicado: (2019)

The CaMKII/MLC1 Axis Confers Ca(2+)-Dependence to Volume-Regulated Anion Channels (VRAC) in Astrocytes
por: Brignone, Maria Stefania, et al.
Publicado: (2022)

Subunit composition of VRAC channels determines substrate specificity and cellular resistance to Pt‐based anti‐cancer drugs
por: Planells‐Cases, Rosa, et al.
Publicado: (2015)

Comparison of UV spectrometry and fluorometry-based methods for quantification of cell-free DNA in red cell components
por: Khetan, Dheeraj, et al.
Publicado: (2019)

Investigation of LRRC8-Mediated Volume-Regulated Anion Currents in Xenopus Oocytes
por: Gaitán-Peñas, Héctor, et al.
Publicado: (2016)

Recent Advances in the Structural Biology of the Volume-Regulated Anion Channel LRRC8
por: Kasuya, Go, et al.
Publicado: (2022)

Quantifying Heterogeneity According to Deformation of the U937 Monocytes and U937-Differentiated Macrophages Using 3D Carbon Dielectrophoresis in Microfluidics
por: Elitas, Meltem, et al.
Publicado: (2020)

Distinct contributions of LRRC8A and its paralogs to the VSOR anion channel from those of the ASOR anion channel
por: Sato-Numata, Kaori, et al.
Publicado: (2016)

Intracellular and extracellular loops of LRRC8 are essential for volume-regulated anion channel function
por: Yamada, Toshiki, et al.
Publicado: (2018)

Biophysics and Structure-Function Relationships of LRRC8-Formed Volume-Regulated Anion Channels
por: König, Benjamin, et al.
Publicado: (2019)

Author Correction: The LRRC8-mediated volume-regulated anion channel is altered in glaucoma
por: Gasull, Xavier, et al.
Publicado: (2020)

Reactive Oxygen Species Imaging in U937 Cells
por: Prasad, Ankush, et al.
Publicado: (2020)

Regulation of the Volume-Regulated Anion Channel Pore-Forming Subunit LRRC8A in the Intrahippocampal Kainic Acid Model of Mesial Temporal Lobe Epilepsy
por: Ghouli, Manolia R., et al.
Publicado: (2023)

The contribution of voltage clamp fluorometry to the understanding of channel and transporter mechanisms
por: Cowgill, John, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_c855hf9lofg1ap1qv9ujq91lls