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Defocused imaging exploits supercritical-angle fluorescence emission for precise axial single molecule localization microscopy

Single molecule localization microscopy (SMLM) is one of the key techniques that break the classical resolution limit in optical imaging. It is based on taking multiple recordings of a sample, each showing only a sparse arrangement of spatially well separated fluorescent molecules which can be local...

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Detalles Bibliográficos
Autores principales: Zelger, Philipp, Bodner, Lisa, Velas, Lukas, Schütz, Gerhard J., Jesacher, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7041438/
https://www.ncbi.nlm.nih.gov/pubmed/32206395
http://dx.doi.org/10.1364/BOE.375678