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Isothermal Titration Calorimetry Enables Rapid Characterization of Enzyme Kinetics and Inhibition for the Human Soluble Epoxide Hydrolase

[Image: see text] Isothermal titration calorimetry (ITC) is conventionally used to acquire thermodynamic data for biological interactions. In recent years, ITC has emerged as a powerful tool to characterize enzyme kinetics. In this study, we have adapted a single-injection method (SIM) to study the...

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Detalles Bibliográficos
Autores principales: Abis, Giancarlo, Pacheco-Gómez, Raúl, Bui, Tam T. T., Conte, Maria R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2019
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7041903/
https://www.ncbi.nlm.nih.gov/pubmed/31660733
http://dx.doi.org/10.1021/acs.analchem.9b01847