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Isothermal Titration Calorimetry Enables Rapid Characterization of Enzyme Kinetics and Inhibition for the Human Soluble Epoxide Hydrolase
[Image: see text] Isothermal titration calorimetry (ITC) is conventionally used to acquire thermodynamic data for biological interactions. In recent years, ITC has emerged as a powerful tool to characterize enzyme kinetics. In this study, we have adapted a single-injection method (SIM) to study the...
Autores principales: | Abis, Giancarlo, Pacheco-Gómez, Raúl, Bui, Tam T. T., Conte, Maria R |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American
Chemical
Society
2019
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7041903/ https://www.ncbi.nlm.nih.gov/pubmed/31660733 http://dx.doi.org/10.1021/acs.analchem.9b01847 |
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