Tendentious effects of automated and manual metagenomic DNA purification protocols on broiler gut microbiome taxonomic profiling

Here, we developed protocols to improve sensitivity, rigor and comparability of 16S rRNA gene amplification-based next-generation sequencing (NGS) results. A thorough study was performed by evaluating extraction efficiency with respect to the yield, purity, fragmentation of the purified DNA, and seq...

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Detalles Bibliográficos
Autores principales: Fidler, Gabor, Tolnai, Emese, Stagel, Aniko, Remenyik, Judit, Stundl, Laszlo, Gal, Ferenc, Biro, Sandor, Paholcsek, Melinda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7042355/
https://www.ncbi.nlm.nih.gov/pubmed/32099013
http://dx.doi.org/10.1038/s41598-020-60304-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7042355/
https://www.ncbi.nlm.nih.gov/pubmed/32099013
http://dx.doi.org/10.1038/s41598-020-60304-y

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