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Cyclic Digestion and Ligation-Mediated PCR Used for Flanking Sequence Walking
Ligation-mediated PCR (LM-PCR) is a classical method for isolating flanking sequences; however, it has a common limitation of reduced success rate owing to the circularization or multimerization of target restriction fragments including the known sequence. To address this limitation, we developed a...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7044209/ https://www.ncbi.nlm.nih.gov/pubmed/32103092 http://dx.doi.org/10.1038/s41598-020-60411-w |