Cargando…

Cyclic Digestion and Ligation-Mediated PCR Used for Flanking Sequence Walking

Ligation-mediated PCR (LM-PCR) is a classical method for isolating flanking sequences; however, it has a common limitation of reduced success rate owing to the circularization or multimerization of target restriction fragments including the known sequence. To address this limitation, we developed a...

Descripción completa

Detalles Bibliográficos
Autores principales: Yu, Dong, Zhou, Tianshun, Sun, Xuewu, Sun, Zhizhong, Sheng, Xiabing, Tan, Yanning, Liu, Ling, Ouyang, Ning, Xu, Ke, Shi, Kaibing, Yuan, Guilong, Ding, Jia, Duan, Meijuan, Yuan, Dingyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7044209/
https://www.ncbi.nlm.nih.gov/pubmed/32103092
http://dx.doi.org/10.1038/s41598-020-60411-w

Ejemplares similares