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A(2B) adenosine receptor inhibition by the dihydropyridine calcium channel blocker nifedipine involves colonic fluid secretion

The adenosine A(2B) receptor is a critical protein in intestinal water secretion. In the present study, we screened compound libraries to identify inhibitors of the A(2B) receptor and evaluated their effect on adenosine-induced intestinal fluid secretion. The screening identified the dihydropyridine...

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Autores principales: Asano, Teita, Noda, Yuto, Tanaka, Ken-Ichiro, Yamakawa, Naoki, Wada, Mitsuhito, Mashimo, Tadaaki, Fukunishi, Yoshifumi, Mizushima, Tohru, Takenaga, Mitsuko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7044278/
https://www.ncbi.nlm.nih.gov/pubmed/32103051
http://dx.doi.org/10.1038/s41598-020-60147-7
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author Asano, Teita
Noda, Yuto
Tanaka, Ken-Ichiro
Yamakawa, Naoki
Wada, Mitsuhito
Mashimo, Tadaaki
Fukunishi, Yoshifumi
Mizushima, Tohru
Takenaga, Mitsuko
author_facet Asano, Teita
Noda, Yuto
Tanaka, Ken-Ichiro
Yamakawa, Naoki
Wada, Mitsuhito
Mashimo, Tadaaki
Fukunishi, Yoshifumi
Mizushima, Tohru
Takenaga, Mitsuko
author_sort Asano, Teita
collection PubMed
description The adenosine A(2B) receptor is a critical protein in intestinal water secretion. In the present study, we screened compound libraries to identify inhibitors of the A(2B) receptor and evaluated their effect on adenosine-induced intestinal fluid secretion. The screening identified the dihydropyridine calcium antagonists nifedipine and nisoldipine. Their respective affinities for the A(2B) receptor (K(i) value) were 886 and 1,399 nM. Nifedipine and nisoldipine, but not amlodipine or nitrendipine, inhibited both calcium mobilization and adenosine-induced cAMP accumulation in cell lines. Moreover, adenosine injection into the lumen significantly increased fluid volume in the colonic loop of wild-type mice but not A(2B) receptor-deficient mice. PSB-1115, a selective A(2B) receptor antagonist, and nifedipine prevented elevated adenosine-stimulated fluid secretion in mice. Our results may provide useful insights into the structure–activity relationship of dihydropyridines for A(2B) receptor. As colonic fluid secretion by adenosine seems to rely predominantly on the A(2B) receptor, nifedipine could be a therapeutic candidate for diarrhoea-related diseases.
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spelling pubmed-70442782020-03-04 A(2B) adenosine receptor inhibition by the dihydropyridine calcium channel blocker nifedipine involves colonic fluid secretion Asano, Teita Noda, Yuto Tanaka, Ken-Ichiro Yamakawa, Naoki Wada, Mitsuhito Mashimo, Tadaaki Fukunishi, Yoshifumi Mizushima, Tohru Takenaga, Mitsuko Sci Rep Article The adenosine A(2B) receptor is a critical protein in intestinal water secretion. In the present study, we screened compound libraries to identify inhibitors of the A(2B) receptor and evaluated their effect on adenosine-induced intestinal fluid secretion. The screening identified the dihydropyridine calcium antagonists nifedipine and nisoldipine. Their respective affinities for the A(2B) receptor (K(i) value) were 886 and 1,399 nM. Nifedipine and nisoldipine, but not amlodipine or nitrendipine, inhibited both calcium mobilization and adenosine-induced cAMP accumulation in cell lines. Moreover, adenosine injection into the lumen significantly increased fluid volume in the colonic loop of wild-type mice but not A(2B) receptor-deficient mice. PSB-1115, a selective A(2B) receptor antagonist, and nifedipine prevented elevated adenosine-stimulated fluid secretion in mice. Our results may provide useful insights into the structure–activity relationship of dihydropyridines for A(2B) receptor. As colonic fluid secretion by adenosine seems to rely predominantly on the A(2B) receptor, nifedipine could be a therapeutic candidate for diarrhoea-related diseases. Nature Publishing Group UK 2020-02-26 /pmc/articles/PMC7044278/ /pubmed/32103051 http://dx.doi.org/10.1038/s41598-020-60147-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Asano, Teita
Noda, Yuto
Tanaka, Ken-Ichiro
Yamakawa, Naoki
Wada, Mitsuhito
Mashimo, Tadaaki
Fukunishi, Yoshifumi
Mizushima, Tohru
Takenaga, Mitsuko
A(2B) adenosine receptor inhibition by the dihydropyridine calcium channel blocker nifedipine involves colonic fluid secretion
title A(2B) adenosine receptor inhibition by the dihydropyridine calcium channel blocker nifedipine involves colonic fluid secretion
title_full A(2B) adenosine receptor inhibition by the dihydropyridine calcium channel blocker nifedipine involves colonic fluid secretion
title_fullStr A(2B) adenosine receptor inhibition by the dihydropyridine calcium channel blocker nifedipine involves colonic fluid secretion
title_full_unstemmed A(2B) adenosine receptor inhibition by the dihydropyridine calcium channel blocker nifedipine involves colonic fluid secretion
title_short A(2B) adenosine receptor inhibition by the dihydropyridine calcium channel blocker nifedipine involves colonic fluid secretion
title_sort a(2b) adenosine receptor inhibition by the dihydropyridine calcium channel blocker nifedipine involves colonic fluid secretion
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7044278/
https://www.ncbi.nlm.nih.gov/pubmed/32103051
http://dx.doi.org/10.1038/s41598-020-60147-7
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