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DMN-Tre Labeling for Detection and High-Content Screening of Compounds against Intracellular Mycobacteria

[Image: see text] 4-N,N-Dimethylamino-1,8-naphthalimide conjugate of trehalose (DMN-Tre) is a fluorogenic dye recently developed as a diagnostic tool for tuberculosis. DMN-Tre selectively labels the mycobacterial cell wall through the Ag85 enzymes. In this work, we disclose a protocol describing the...

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Detalles Bibliográficos
Autores principales: Sahile, Henok A., Rens, Celine, Shapira, Tirosh, Andersen, Raymond J., Av-Gay, Yossef
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7045496/
https://www.ncbi.nlm.nih.gov/pubmed/32118181
http://dx.doi.org/10.1021/acsomega.9b04173