Reticulon proteins modulate autophagy of the endoplasmic reticulum in maize endosperm

Reticulon (Rtn) proteins shape tubular domains of the endoplasmic reticulum (ER), and in some cases are autophagy receptors for selective ER turnover. We have found that maize Rtn1 and Rtn2 control ER homeostasis and autophagic flux in endosperm aleurone cells, where the ER accumulates lipid droplet...

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Autores principales: Zhang, Xiaoguo, Ding, Xinxin, Marshall, Richard Scott, Paez-Valencia, Julio, Lacey, Patrick, Vierstra, Richard David, Otegui, Marisa S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7046470/
https://www.ncbi.nlm.nih.gov/pubmed/32011236
http://dx.doi.org/10.7554/eLife.51918
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author Zhang, Xiaoguo
Ding, Xinxin
Marshall, Richard Scott
Paez-Valencia, Julio
Lacey, Patrick
Vierstra, Richard David
Otegui, Marisa S
author_facet Zhang, Xiaoguo
Ding, Xinxin
Marshall, Richard Scott
Paez-Valencia, Julio
Lacey, Patrick
Vierstra, Richard David
Otegui, Marisa S
author_sort Zhang, Xiaoguo
collection PubMed
description Reticulon (Rtn) proteins shape tubular domains of the endoplasmic reticulum (ER), and in some cases are autophagy receptors for selective ER turnover. We have found that maize Rtn1 and Rtn2 control ER homeostasis and autophagic flux in endosperm aleurone cells, where the ER accumulates lipid droplets and synthesizes storage protein accretions metabolized during germination. Maize Rtn1 and Rtn2 are expressed in the endosperm, localize to the ER, and re-model ER architecture in a dose-dependent manner. Rtn1 and Rtn2 interact with Atg8a using four Atg8-interacting motifs (AIMs) located at the C-terminus, cytoplasmic loop, and within the transmembrane segments. Binding between Rtn2 and Atg8 is elevated upon ER stress. Maize rtn2 mutants display increased autophagy and up-regulation of an ER stress-responsive chaperone. We propose that maize Rtn1 and Rtn2 act as receptors for autophagy-mediated ER turnover, and thus are critical for ER homeostasis and suppression of ER stress.
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spelling pubmed-70464702020-03-02 Reticulon proteins modulate autophagy of the endoplasmic reticulum in maize endosperm Zhang, Xiaoguo Ding, Xinxin Marshall, Richard Scott Paez-Valencia, Julio Lacey, Patrick Vierstra, Richard David Otegui, Marisa S eLife Cell Biology Reticulon (Rtn) proteins shape tubular domains of the endoplasmic reticulum (ER), and in some cases are autophagy receptors for selective ER turnover. We have found that maize Rtn1 and Rtn2 control ER homeostasis and autophagic flux in endosperm aleurone cells, where the ER accumulates lipid droplets and synthesizes storage protein accretions metabolized during germination. Maize Rtn1 and Rtn2 are expressed in the endosperm, localize to the ER, and re-model ER architecture in a dose-dependent manner. Rtn1 and Rtn2 interact with Atg8a using four Atg8-interacting motifs (AIMs) located at the C-terminus, cytoplasmic loop, and within the transmembrane segments. Binding between Rtn2 and Atg8 is elevated upon ER stress. Maize rtn2 mutants display increased autophagy and up-regulation of an ER stress-responsive chaperone. We propose that maize Rtn1 and Rtn2 act as receptors for autophagy-mediated ER turnover, and thus are critical for ER homeostasis and suppression of ER stress. eLife Sciences Publications, Ltd 2020-02-03 /pmc/articles/PMC7046470/ /pubmed/32011236 http://dx.doi.org/10.7554/eLife.51918 Text en © 2020, Zhang et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
Zhang, Xiaoguo
Ding, Xinxin
Marshall, Richard Scott
Paez-Valencia, Julio
Lacey, Patrick
Vierstra, Richard David
Otegui, Marisa S
Reticulon proteins modulate autophagy of the endoplasmic reticulum in maize endosperm
title Reticulon proteins modulate autophagy of the endoplasmic reticulum in maize endosperm
title_full Reticulon proteins modulate autophagy of the endoplasmic reticulum in maize endosperm
title_fullStr Reticulon proteins modulate autophagy of the endoplasmic reticulum in maize endosperm
title_full_unstemmed Reticulon proteins modulate autophagy of the endoplasmic reticulum in maize endosperm
title_short Reticulon proteins modulate autophagy of the endoplasmic reticulum in maize endosperm
title_sort reticulon proteins modulate autophagy of the endoplasmic reticulum in maize endosperm
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7046470/
https://www.ncbi.nlm.nih.gov/pubmed/32011236
http://dx.doi.org/10.7554/eLife.51918
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