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A method to dissolve 3-MCPD mono- and di-esters in aqueous cell culture media
Fatty acid esters of 3-monochloropropane-1,2-diol (3-MCPD) are chemical contaminants found in a wide range of edible oils that are thermally processed during industrial manufacturing of infant formula and other lipid-containing foods in the United States. Rodent studies have unequivocally demonstrat...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7046810/ https://www.ncbi.nlm.nih.gov/pubmed/32140439 http://dx.doi.org/10.1016/j.mex.2019.100774 |
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author | Mapa, Mapa S.T. Araujo, Magali Zhao, Yang Flynn, Thomas Sprando, Jessica Wiesenfeld, Paddy Sprando, Robert L. Mossoba, Miriam E. |
author_facet | Mapa, Mapa S.T. Araujo, Magali Zhao, Yang Flynn, Thomas Sprando, Jessica Wiesenfeld, Paddy Sprando, Robert L. Mossoba, Miriam E. |
author_sort | Mapa, Mapa S.T. |
collection | PubMed |
description | Fatty acid esters of 3-monochloropropane-1,2-diol (3-MCPD) are chemical contaminants found in a wide range of edible oils that are thermally processed during industrial manufacturing of infant formula and other lipid-containing foods in the United States. Rodent studies have unequivocally demonstrated a plethora of in vivo toxicological effects including reproductive, neurological and renal dysfunction. To determine if similar effects are observed in human organ systems, in vitro studies using human cell lines are conducted to assess concordance of the data collected. One limitation to performing such in vitro research is the extremely high hydrophobicity of 3-MCPD esters; dissolving them into aqueous cell culture media is a tremendous challenge. To address this obstacle, we developed a simple protocol to circumvent the immiscibility of 3-MCPD esters and their corresponding free fatty acids into aqueous cell culture media in order to assess the effect of these esters on epithelial cells of kidney origin in vitro. |
format | Online Article Text |
id | pubmed-7046810 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-70468102020-03-05 A method to dissolve 3-MCPD mono- and di-esters in aqueous cell culture media Mapa, Mapa S.T. Araujo, Magali Zhao, Yang Flynn, Thomas Sprando, Jessica Wiesenfeld, Paddy Sprando, Robert L. Mossoba, Miriam E. MethodsX Pharmacology, Toxicology and Pharmaceutical Science Fatty acid esters of 3-monochloropropane-1,2-diol (3-MCPD) are chemical contaminants found in a wide range of edible oils that are thermally processed during industrial manufacturing of infant formula and other lipid-containing foods in the United States. Rodent studies have unequivocally demonstrated a plethora of in vivo toxicological effects including reproductive, neurological and renal dysfunction. To determine if similar effects are observed in human organ systems, in vitro studies using human cell lines are conducted to assess concordance of the data collected. One limitation to performing such in vitro research is the extremely high hydrophobicity of 3-MCPD esters; dissolving them into aqueous cell culture media is a tremendous challenge. To address this obstacle, we developed a simple protocol to circumvent the immiscibility of 3-MCPD esters and their corresponding free fatty acids into aqueous cell culture media in order to assess the effect of these esters on epithelial cells of kidney origin in vitro. Elsevier 2019-12-17 /pmc/articles/PMC7046810/ /pubmed/32140439 http://dx.doi.org/10.1016/j.mex.2019.100774 Text en © 2019 Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Pharmacology, Toxicology and Pharmaceutical Science Mapa, Mapa S.T. Araujo, Magali Zhao, Yang Flynn, Thomas Sprando, Jessica Wiesenfeld, Paddy Sprando, Robert L. Mossoba, Miriam E. A method to dissolve 3-MCPD mono- and di-esters in aqueous cell culture media |
title | A method to dissolve 3-MCPD mono- and di-esters in aqueous cell culture media |
title_full | A method to dissolve 3-MCPD mono- and di-esters in aqueous cell culture media |
title_fullStr | A method to dissolve 3-MCPD mono- and di-esters in aqueous cell culture media |
title_full_unstemmed | A method to dissolve 3-MCPD mono- and di-esters in aqueous cell culture media |
title_short | A method to dissolve 3-MCPD mono- and di-esters in aqueous cell culture media |
title_sort | method to dissolve 3-mcpd mono- and di-esters in aqueous cell culture media |
topic | Pharmacology, Toxicology and Pharmaceutical Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7046810/ https://www.ncbi.nlm.nih.gov/pubmed/32140439 http://dx.doi.org/10.1016/j.mex.2019.100774 |
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