Antibacterial Activity of Lidah Mertua (Sansevieria Trifasciata Prain.) Leaves Extract on Escherichia coli and Staphylococcus aureus
BACKGROUND: Infection is the most common diseases in developing country, including Indonesia. Bacteria that often causes infection is Escherichia coli and Staphylococcus aureus. One of the traditional plants that can be used as an antibacterial is lidah mertua. AIM: The purpose of this study was to...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Republic of Macedonia
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7048363/ https://www.ncbi.nlm.nih.gov/pubmed/32127997 http://dx.doi.org/10.3889/oamjms.2019.525 |
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author | Febriani, Yessi Mierza, Vriezka Handayani, Novi Putri Surismayanti, Surismayanti Ginting, Ibrenaita |
author_facet | Febriani, Yessi Mierza, Vriezka Handayani, Novi Putri Surismayanti, Surismayanti Ginting, Ibrenaita |
author_sort | Febriani, Yessi |
collection | PubMed |
description | BACKGROUND: Infection is the most common diseases in developing country, including Indonesia. Bacteria that often causes infection is Escherichia coli and Staphylococcus aureus. One of the traditional plants that can be used as an antibacterial is lidah mertua. AIM: The purpose of this study was to find out the profile of chemical compounds by thin layer chromatography method and determine the antibacterial activity of Lidah Mertua leaves by in vitro. METHODS: This research conducted an experimentally using non-polar, semipolar, and polar as solvents to get extract against E. coli and S. aureus as bacterial testing. The antibacterial activity using agar diffusion method to get minimum inhibitory concentration (MIC). RESULTS: The result of the research on thin layer chromatography showed that the compounds contained in the Lidah Mertua leaves were polifenol, steroids and alkaloids. The data obtained were tabulated and analysed descriptively. The antibacterial activity show that n-hexane extract does not provide inhibitory activity. MIC value show that aethyl acetate extract of lidah mertua leaves inhibited the growth of E. coli and S. aureus at concentration 50 mg/mL and 25 mg/mL with diameters of inhibition zone is 8.50 mm and 8.20 mm and methanol extract of lidah mertua leaves inhibited the growth of E. coli and S. aureus at concentration 12.5 mg/mL and 25 mg/mL with diameters of inhibition zone is 8.46 mm and 8.32 mm. CONCLUSION: The profile of chemical compounds by thin layer chromatography method showed that the compounds contained in the Lidah Mertua leaves were polifenol, steroids and alkaloids. The antibacterial activity show that n-hexane extract does not provide inhibitory activity, but aethyl acetate extract of lidah mertua leaves inhibited the growth of E. coli and S. aureus. |
format | Online Article Text |
id | pubmed-7048363 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Republic of Macedonia |
record_format | MEDLINE/PubMed |
spelling | pubmed-70483632020-03-03 Antibacterial Activity of Lidah Mertua (Sansevieria Trifasciata Prain.) Leaves Extract on Escherichia coli and Staphylococcus aureus Febriani, Yessi Mierza, Vriezka Handayani, Novi Putri Surismayanti, Surismayanti Ginting, Ibrenaita Open Access Maced J Med Sci Herbal Medicine in Pharmaceutical and Clinical Sciences BACKGROUND: Infection is the most common diseases in developing country, including Indonesia. Bacteria that often causes infection is Escherichia coli and Staphylococcus aureus. One of the traditional plants that can be used as an antibacterial is lidah mertua. AIM: The purpose of this study was to find out the profile of chemical compounds by thin layer chromatography method and determine the antibacterial activity of Lidah Mertua leaves by in vitro. METHODS: This research conducted an experimentally using non-polar, semipolar, and polar as solvents to get extract against E. coli and S. aureus as bacterial testing. The antibacterial activity using agar diffusion method to get minimum inhibitory concentration (MIC). RESULTS: The result of the research on thin layer chromatography showed that the compounds contained in the Lidah Mertua leaves were polifenol, steroids and alkaloids. The data obtained were tabulated and analysed descriptively. The antibacterial activity show that n-hexane extract does not provide inhibitory activity. MIC value show that aethyl acetate extract of lidah mertua leaves inhibited the growth of E. coli and S. aureus at concentration 50 mg/mL and 25 mg/mL with diameters of inhibition zone is 8.50 mm and 8.20 mm and methanol extract of lidah mertua leaves inhibited the growth of E. coli and S. aureus at concentration 12.5 mg/mL and 25 mg/mL with diameters of inhibition zone is 8.46 mm and 8.32 mm. CONCLUSION: The profile of chemical compounds by thin layer chromatography method showed that the compounds contained in the Lidah Mertua leaves were polifenol, steroids and alkaloids. The antibacterial activity show that n-hexane extract does not provide inhibitory activity, but aethyl acetate extract of lidah mertua leaves inhibited the growth of E. coli and S. aureus. Republic of Macedonia 2019-11-14 /pmc/articles/PMC7048363/ /pubmed/32127997 http://dx.doi.org/10.3889/oamjms.2019.525 Text en Copyright: © 2019 Yessi Febriani, Vriezka Mierza, Novi Putri Handayani, Surismayanti Surismayanti, Ibrenaita Ginting. http://creativecommons.org/licenses/CC BY-NC/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0) |
spellingShingle | Herbal Medicine in Pharmaceutical and Clinical Sciences Febriani, Yessi Mierza, Vriezka Handayani, Novi Putri Surismayanti, Surismayanti Ginting, Ibrenaita Antibacterial Activity of Lidah Mertua (Sansevieria Trifasciata Prain.) Leaves Extract on Escherichia coli and Staphylococcus aureus |
title | Antibacterial Activity of Lidah Mertua (Sansevieria Trifasciata Prain.) Leaves Extract on Escherichia coli and Staphylococcus aureus |
title_full | Antibacterial Activity of Lidah Mertua (Sansevieria Trifasciata Prain.) Leaves Extract on Escherichia coli and Staphylococcus aureus |
title_fullStr | Antibacterial Activity of Lidah Mertua (Sansevieria Trifasciata Prain.) Leaves Extract on Escherichia coli and Staphylococcus aureus |
title_full_unstemmed | Antibacterial Activity of Lidah Mertua (Sansevieria Trifasciata Prain.) Leaves Extract on Escherichia coli and Staphylococcus aureus |
title_short | Antibacterial Activity of Lidah Mertua (Sansevieria Trifasciata Prain.) Leaves Extract on Escherichia coli and Staphylococcus aureus |
title_sort | antibacterial activity of lidah mertua (sansevieria trifasciata prain.) leaves extract on escherichia coli and staphylococcus aureus |
topic | Herbal Medicine in Pharmaceutical and Clinical Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7048363/ https://www.ncbi.nlm.nih.gov/pubmed/32127997 http://dx.doi.org/10.3889/oamjms.2019.525 |
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