In vitro ribosome synthesis and evolution through ribosome display

Directed evolution of the ribosome for expanded substrate incorporation and novel functions is challenging because the requirement of cell viability limits the mutations that can be made. Here we address this challenge by combining cell-free synthesis and assembly of translationally competent riboso...

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Detalles Bibliográficos
Autores principales: Hammerling, Michael J., Fritz, Brian R., Yoesep, Danielle J., Kim, Do Soon, Carlson, Erik D., Jewett, Michael C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7048773/
https://www.ncbi.nlm.nih.gov/pubmed/32111839
http://dx.doi.org/10.1038/s41467-020-14705-2