Systematic optimization of square-wave electroporation conditions for bovine primary fibroblasts

BACKGROUND: Gene transfer by electroporation is an established method for the non-viral mediated transfection of mammalian cells. Primary cells pose a particular challenge for electroporation-mediated gene transfer, since they are more vulnerable than immortalized cells, and have a limited prolifera...

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Detalles Bibliográficos
Autores principales: Hyder, Iqbal, Eghbalsaied, Shahin, Kues, Wilfried A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7049184/
https://www.ncbi.nlm.nih.gov/pubmed/32111153
http://dx.doi.org/10.1186/s12860-020-00254-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7049184/
https://www.ncbi.nlm.nih.gov/pubmed/32111153
http://dx.doi.org/10.1186/s12860-020-00254-5

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