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Systematic optimization of square-wave electroporation conditions for bovine primary fibroblasts
BACKGROUND: Gene transfer by electroporation is an established method for the non-viral mediated transfection of mammalian cells. Primary cells pose a particular challenge for electroporation-mediated gene transfer, since they are more vulnerable than immortalized cells, and have a limited prolifera...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2020
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7049184/ https://www.ncbi.nlm.nih.gov/pubmed/32111153 http://dx.doi.org/10.1186/s12860-020-00254-5 |