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Tumor-educated B cells promote renal cancer metastasis via inducing the IL-1β/HIF-2α/Notch1 signals

While B cells in the tumor microenvironment (TME) might play important roles in cancer progression, their impacts on the renal cell carcinoma (RCC) metastasis remained unclear, which drew our attention to further explore. We found that RCC tissues could recruit more B cells than the surrounding norm...

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Autores principales: Li, Saiyang, Huang, Chi, Hu, Guanghui, Ma, Junjie, Chen, Yonghui, Zhang, Jin, Huang, Yiran, Zheng, Junhua, Xue, Wei, Xu, Yunfei, Zhai, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7052134/
https://www.ncbi.nlm.nih.gov/pubmed/32123166
http://dx.doi.org/10.1038/s41419-020-2355-x
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author Li, Saiyang
Huang, Chi
Hu, Guanghui
Ma, Junjie
Chen, Yonghui
Zhang, Jin
Huang, Yiran
Zheng, Junhua
Xue, Wei
Xu, Yunfei
Zhai, Wei
author_facet Li, Saiyang
Huang, Chi
Hu, Guanghui
Ma, Junjie
Chen, Yonghui
Zhang, Jin
Huang, Yiran
Zheng, Junhua
Xue, Wei
Xu, Yunfei
Zhai, Wei
author_sort Li, Saiyang
collection PubMed
description While B cells in the tumor microenvironment (TME) might play important roles in cancer progression, their impacts on the renal cell carcinoma (RCC) metastasis remained unclear, which drew our attention to further explore. We found that RCC tissues could recruit more B cells than the surrounding normal renal tissues from human clinical RCC samples. Wound healing assay, transwell assay and 3D invasion assays demonstrated that recruited B cells, also known as tumor-educated B cells (TEB), could significantly increase the RCC cell migration and invasion. In addition, in vivo data from xenograft RCC mouse model also confirmed that TEB could enhance RCC cell invasive and metastatic capability. Mechanism dissection revealed that TEB activated IL-1β/HIF-2α signals in RCC cells that could induce the downstream Notch1 signaling pathway. The above results demonstrated the key roles of TEB within renal cancer associated tumor microenvironment were metastasis-promotor and might help us to develop the potential therapies via targeting these newly identified IL-1β/HIF-2α/Notch1 signals in RCC progression.
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spelling pubmed-70521342020-03-05 Tumor-educated B cells promote renal cancer metastasis via inducing the IL-1β/HIF-2α/Notch1 signals Li, Saiyang Huang, Chi Hu, Guanghui Ma, Junjie Chen, Yonghui Zhang, Jin Huang, Yiran Zheng, Junhua Xue, Wei Xu, Yunfei Zhai, Wei Cell Death Dis Article While B cells in the tumor microenvironment (TME) might play important roles in cancer progression, their impacts on the renal cell carcinoma (RCC) metastasis remained unclear, which drew our attention to further explore. We found that RCC tissues could recruit more B cells than the surrounding normal renal tissues from human clinical RCC samples. Wound healing assay, transwell assay and 3D invasion assays demonstrated that recruited B cells, also known as tumor-educated B cells (TEB), could significantly increase the RCC cell migration and invasion. In addition, in vivo data from xenograft RCC mouse model also confirmed that TEB could enhance RCC cell invasive and metastatic capability. Mechanism dissection revealed that TEB activated IL-1β/HIF-2α signals in RCC cells that could induce the downstream Notch1 signaling pathway. The above results demonstrated the key roles of TEB within renal cancer associated tumor microenvironment were metastasis-promotor and might help us to develop the potential therapies via targeting these newly identified IL-1β/HIF-2α/Notch1 signals in RCC progression. Nature Publishing Group UK 2020-03-02 /pmc/articles/PMC7052134/ /pubmed/32123166 http://dx.doi.org/10.1038/s41419-020-2355-x Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Li, Saiyang
Huang, Chi
Hu, Guanghui
Ma, Junjie
Chen, Yonghui
Zhang, Jin
Huang, Yiran
Zheng, Junhua
Xue, Wei
Xu, Yunfei
Zhai, Wei
Tumor-educated B cells promote renal cancer metastasis via inducing the IL-1β/HIF-2α/Notch1 signals
title Tumor-educated B cells promote renal cancer metastasis via inducing the IL-1β/HIF-2α/Notch1 signals
title_full Tumor-educated B cells promote renal cancer metastasis via inducing the IL-1β/HIF-2α/Notch1 signals
title_fullStr Tumor-educated B cells promote renal cancer metastasis via inducing the IL-1β/HIF-2α/Notch1 signals
title_full_unstemmed Tumor-educated B cells promote renal cancer metastasis via inducing the IL-1β/HIF-2α/Notch1 signals
title_short Tumor-educated B cells promote renal cancer metastasis via inducing the IL-1β/HIF-2α/Notch1 signals
title_sort tumor-educated b cells promote renal cancer metastasis via inducing the il-1β/hif-2α/notch1 signals
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7052134/
https://www.ncbi.nlm.nih.gov/pubmed/32123166
http://dx.doi.org/10.1038/s41419-020-2355-x
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