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Multi-target immunofluorescence by separation of antibody cross-labelling via spectral-FLIM-FRET

In biomedical research, indirect immunofluorescence labelling by use of primary and secondary antibodies is central for revealing the spatial distribution of multiple cellular antigens. However, labelling is regularly restricted to few antigens since species variation of primary and corresponding se...

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Detalles Bibliográficos
Autores principales: Rohilla, Sumeet, Krämer, Benedikt, Koberling, Felix, Gregor, Ingo, Hocke, Andreas C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7052234/
https://www.ncbi.nlm.nih.gov/pubmed/32123277
http://dx.doi.org/10.1038/s41598-020-60877-8