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Multi-target immunofluorescence by separation of antibody cross-labelling via spectral-FLIM-FRET
In biomedical research, indirect immunofluorescence labelling by use of primary and secondary antibodies is central for revealing the spatial distribution of multiple cellular antigens. However, labelling is regularly restricted to few antigens since species variation of primary and corresponding se...
Autores principales: | Rohilla, Sumeet, Krämer, Benedikt, Koberling, Felix, Gregor, Ingo, Hocke, Andreas C. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7052234/ https://www.ncbi.nlm.nih.gov/pubmed/32123277 http://dx.doi.org/10.1038/s41598-020-60877-8 |
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