gscreend: modelling asymmetric count ratios in CRISPR screens to decrease experiment size and improve phenotype detection

Pooled CRISPR screens are a powerful tool to probe genotype-phenotype relationships at genome-wide scale. However, criteria for optimal design are missing, and it remains unclear how experimental parameters affect results. Here, we report that random decreases in gRNA abundance are more likely than...

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Detalles Bibliográficos
Autores principales: Imkeller, Katharina, Ambrosi, Giulia, Boutros, Michael, Huber, Wolfgang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7052974/
https://www.ncbi.nlm.nih.gov/pubmed/32122365
http://dx.doi.org/10.1186/s13059-020-1939-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7052974/
https://www.ncbi.nlm.nih.gov/pubmed/32122365
http://dx.doi.org/10.1186/s13059-020-1939-1

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