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A simple and affordable kinetic assay of nucleic acids with SYBR Gold gel staining
Labeling substrates or products are paramount in determining enzymatic kinetic parameters. Several options are available; many laboratories use either radioactive or fluorescent labeling because of their high sensitivity. However, those methods have their own drawbacks such as half-life decay, expen...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053750/ https://www.ncbi.nlm.nih.gov/pubmed/32126098 http://dx.doi.org/10.1371/journal.pone.0229527 |