New approach for membrane protein reconstitution into peptidiscs and basis for their adaptability to different proteins

Previously we introduced peptidiscs as an alternative to detergents to stabilize membrane proteins in solution (Carlson et al., 2018). Here, we present ‘on-gradient’ reconstitution, a new gentle approach for the reconstitution of labile membrane-protein complexes, and used it to reconstitute Rhodoba...

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Detalles Bibliográficos
Autores principales: Angiulli, Gabriella, Dhupar, Harveer Singh, Suzuki, Hiroshi, Wason, Irvinder Singh, Duong Van Hoa, Franck, Walz, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2020
Materias:
Biochemistry and Chemical Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053995/
https://www.ncbi.nlm.nih.gov/pubmed/32125274
http://dx.doi.org/10.7554/eLife.53530

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053995/
https://www.ncbi.nlm.nih.gov/pubmed/32125274
http://dx.doi.org/10.7554/eLife.53530

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