Structural and functional comparison of fumarylacetoacetate domain containing protein 1 in human and mouse

FAH domain containing protein 1 (FAHD1) is a mammalian mitochondrial protein, displaying bifunctionality as acylpyruvate hydrolase (ApH) and oxaloacetate decarboxylase (ODx) activity. We report the crystal structure of mouse FAHD1 and structural mapping of the active site of mouse FAHD1. Despite hig...

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Autores principales: Weiss, Alexander K.H., Naschberger, Andreas, Cappuccio, Elia, Metzger, Christina, Mottes, Lorenza, Holzknecht, Max, von Velsen, Jill, Bowler, Matthew W., Rupp, Bernhard, Jansen-Dürr, Pidder
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2020
Materias:
Chemical Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7056447/
https://www.ncbi.nlm.nih.gov/pubmed/32068790
http://dx.doi.org/10.1042/BSR20194431
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author Weiss, Alexander K.H.
Naschberger, Andreas
Cappuccio, Elia
Metzger, Christina
Mottes, Lorenza
Holzknecht, Max
von Velsen, Jill
Bowler, Matthew W.
Rupp, Bernhard
Jansen-Dürr, Pidder
author_facet Weiss, Alexander K.H.
Naschberger, Andreas
Cappuccio, Elia
Metzger, Christina
Mottes, Lorenza
Holzknecht, Max
von Velsen, Jill
Bowler, Matthew W.
Rupp, Bernhard
Jansen-Dürr, Pidder
author_sort Weiss, Alexander K.H.
collection PubMed
description FAH domain containing protein 1 (FAHD1) is a mammalian mitochondrial protein, displaying bifunctionality as acylpyruvate hydrolase (ApH) and oxaloacetate decarboxylase (ODx) activity. We report the crystal structure of mouse FAHD1 and structural mapping of the active site of mouse FAHD1. Despite high structural similarity with human FAHD1, a rabbit monoclonal antibody (RabMab) could be produced that is able to recognize mouse FAHD1, but not the human form, whereas a polyclonal antibody recognized both proteins. Epitope mapping in combination with our deposited crystal structures revealed that the epitope overlaps with a reported SIRT3 deacetylation site in mouse FAHD1.
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spelling pubmed-70564472020-03-11 Structural and functional comparison of fumarylacetoacetate domain containing protein 1 in human and mouse Weiss, Alexander K.H. Naschberger, Andreas Cappuccio, Elia Metzger, Christina Mottes, Lorenza Holzknecht, Max von Velsen, Jill Bowler, Matthew W. Rupp, Bernhard Jansen-Dürr, Pidder Biosci Rep Chemical Biology FAH domain containing protein 1 (FAHD1) is a mammalian mitochondrial protein, displaying bifunctionality as acylpyruvate hydrolase (ApH) and oxaloacetate decarboxylase (ODx) activity. We report the crystal structure of mouse FAHD1 and structural mapping of the active site of mouse FAHD1. Despite high structural similarity with human FAHD1, a rabbit monoclonal antibody (RabMab) could be produced that is able to recognize mouse FAHD1, but not the human form, whereas a polyclonal antibody recognized both proteins. Epitope mapping in combination with our deposited crystal structures revealed that the epitope overlaps with a reported SIRT3 deacetylation site in mouse FAHD1. Portland Press Ltd. 2020-03-04 /pmc/articles/PMC7056447/ /pubmed/32068790 http://dx.doi.org/10.1042/BSR20194431 Text en © 2020 The Author(s). https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY).
spellingShingle Chemical Biology
Weiss, Alexander K.H.
Naschberger, Andreas
Cappuccio, Elia
Metzger, Christina
Mottes, Lorenza
Holzknecht, Max
von Velsen, Jill
Bowler, Matthew W.
Rupp, Bernhard
Jansen-Dürr, Pidder
Structural and functional comparison of fumarylacetoacetate domain containing protein 1 in human and mouse
title Structural and functional comparison of fumarylacetoacetate domain containing protein 1 in human and mouse
title_full Structural and functional comparison of fumarylacetoacetate domain containing protein 1 in human and mouse
title_fullStr Structural and functional comparison of fumarylacetoacetate domain containing protein 1 in human and mouse
title_full_unstemmed Structural and functional comparison of fumarylacetoacetate domain containing protein 1 in human and mouse
title_short Structural and functional comparison of fumarylacetoacetate domain containing protein 1 in human and mouse
title_sort structural and functional comparison of fumarylacetoacetate domain containing protein 1 in human and mouse
topic Chemical Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7056447/
https://www.ncbi.nlm.nih.gov/pubmed/32068790
http://dx.doi.org/10.1042/BSR20194431
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