A Single “All-in-One” Helper-Dependent Adenovirus to Deliver Donor DNA and CRISPR/Cas9 for Efficient Homology-Directed Repair

In this study, we developed a single helper-dependent adenovirus (HDAd) to deliver all of the components (donor DNA, CRISPR-associated protein 9 [Cas9], and guide RNA [gRNA]) needed to achieve high-efficiency gene targeting and homology-directed repair in transduced cells. We show that these “all-in...

Descripción completa

Detalles Bibliográficos
Autores principales: Palmer, Donna J., Turner, Dustin L., Ng, Philip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7058846/
https://www.ncbi.nlm.nih.gov/pubmed/32154329
http://dx.doi.org/10.1016/j.omtm.2020.01.014

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7058846/
https://www.ncbi.nlm.nih.gov/pubmed/32154329
http://dx.doi.org/10.1016/j.omtm.2020.01.014

Ejemplares similares