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Escherichia coli σ(70) promoters allow expression rate control at the cellular level in genome-integrated expression systems

BACKGROUND: The genome-integrated T7 expression system offers significant advantages, in terms of productivity and product quality, even when expressing the gene of interest (GOI) from a single copy. Compared to plasmid-based expression systems, this system does not incur a plasmid-mediated metaboli...

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Detalles Bibliográficos
Autores principales: Schuller, Artur, Cserjan-Puschmann, Monika, Tauer, Christopher, Jarmer, Johanna, Wagenknecht, Martin, Reinisch, Daniela, Grabherr, Reingard, Striedner, Gerald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7059391/
https://www.ncbi.nlm.nih.gov/pubmed/32138729
http://dx.doi.org/10.1186/s12934-020-01311-6