Decontamination of ambient RNA in single-cell RNA-seq with DecontX

Droplet-based microfluidic devices have become widely used to perform single-cell RNA sequencing (scRNA-seq). However, ambient RNA present in the cell suspension can be aberrantly counted along with a cell’s native mRNA and result in cross-contamination of transcripts between different cell populati...

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Detalles Bibliográficos
Autores principales: Yang, Shiyi, Corbett, Sean E., Koga, Yusuke, Wang, Zhe, Johnson, W Evan, Yajima, Masanao, Campbell, Joshua D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7059395/
https://www.ncbi.nlm.nih.gov/pubmed/32138770
http://dx.doi.org/10.1186/s13059-020-1950-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7059395/
https://www.ncbi.nlm.nih.gov/pubmed/32138770
http://dx.doi.org/10.1186/s13059-020-1950-6

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