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Effects of Enzyme Loading and Immobilization Conditions on the Catalytic Features of Lipase From Pseudomonas fluorescens Immobilized on Octyl-Agarose Beads

The lipase from Pseudomonas fluorescens (PFL) has been immobilized on octyl-agarose beads under 16 different conditions (varying pH, ionic strength, buffer, adding some additives) at two different loadings, 1 and 60 mg of enzyme/g of support with the objective of check if this can alter the biocatal...

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Detalles Bibliográficos
Autores principales: Arana-Peña, Sara, Rios, Nathalia S., Carballares, Diego, Mendez-Sanchez, Carmen, Lokha, Yuliya, Gonçalves, Luciana R. B., Fernandez-Lafuente, Roberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7059646/
https://www.ncbi.nlm.nih.gov/pubmed/32181245
http://dx.doi.org/10.3389/fbioe.2020.00036