Multiplexed CRISPR technologies for gene editing and transcriptional regulation

Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are expressed at once, have facilitated powerful biological engineering applications, vastly enhancing the scope and efficiencies of genetic editing and transcriptional regulation. In this review, we discuss multiplexed CRISPR t...

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Autores principales: McCarty, Nicholas S., Graham, Alicia E., Studená, Lucie, Ledesma-Amaro, Rodrigo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Review Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7062760/
https://www.ncbi.nlm.nih.gov/pubmed/32152313
http://dx.doi.org/10.1038/s41467-020-15053-x
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author McCarty, Nicholas S.
Graham, Alicia E.
Studená, Lucie
Ledesma-Amaro, Rodrigo
author_facet McCarty, Nicholas S.
Graham, Alicia E.
Studená, Lucie
Ledesma-Amaro, Rodrigo
author_sort McCarty, Nicholas S.
collection PubMed
description Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are expressed at once, have facilitated powerful biological engineering applications, vastly enhancing the scope and efficiencies of genetic editing and transcriptional regulation. In this review, we discuss multiplexed CRISPR technologies and describe methods for the assembly, expression and processing of synthetic guide RNA arrays in vivo. Applications that benefit from multiplexed CRISPR technologies, including cellular recorders, genetic circuits, biosensors, combinatorial genetic perturbations, large-scale genome engineering and the rewiring of metabolic pathways, are highlighted. We also offer a glimpse of emerging challenges and emphasize experimental considerations for future studies.
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spelling pubmed-70627602020-03-18 Multiplexed CRISPR technologies for gene editing and transcriptional regulation McCarty, Nicholas S. Graham, Alicia E. Studená, Lucie Ledesma-Amaro, Rodrigo Nat Commun Review Article Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are expressed at once, have facilitated powerful biological engineering applications, vastly enhancing the scope and efficiencies of genetic editing and transcriptional regulation. In this review, we discuss multiplexed CRISPR technologies and describe methods for the assembly, expression and processing of synthetic guide RNA arrays in vivo. Applications that benefit from multiplexed CRISPR technologies, including cellular recorders, genetic circuits, biosensors, combinatorial genetic perturbations, large-scale genome engineering and the rewiring of metabolic pathways, are highlighted. We also offer a glimpse of emerging challenges and emphasize experimental considerations for future studies. Nature Publishing Group UK 2020-03-09 /pmc/articles/PMC7062760/ /pubmed/32152313 http://dx.doi.org/10.1038/s41467-020-15053-x Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Review Article
McCarty, Nicholas S.
Graham, Alicia E.
Studená, Lucie
Ledesma-Amaro, Rodrigo
Multiplexed CRISPR technologies for gene editing and transcriptional regulation
title Multiplexed CRISPR technologies for gene editing and transcriptional regulation
title_full Multiplexed CRISPR technologies for gene editing and transcriptional regulation
title_fullStr Multiplexed CRISPR technologies for gene editing and transcriptional regulation
title_full_unstemmed Multiplexed CRISPR technologies for gene editing and transcriptional regulation
title_short Multiplexed CRISPR technologies for gene editing and transcriptional regulation
title_sort multiplexed crispr technologies for gene editing and transcriptional regulation
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7062760/
https://www.ncbi.nlm.nih.gov/pubmed/32152313
http://dx.doi.org/10.1038/s41467-020-15053-x
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