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CATS: Cas9-assisted tag switching. A high-throughput method for exchanging genomic peptide tags in yeast

BACKGROUND: The creation of arrays of yeast strains each encoding a different protein with constant tags is a powerful method for understanding how genes and their proteins control cell function. As genetic tools become more sophisticated there is a need to create custom libraries encoding proteins...

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Detalles Bibliográficos
Autores principales: Berry, Lisa K., Thomas, Grace Heredge, Thorpe, Peter H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7063721/
https://www.ncbi.nlm.nih.gov/pubmed/32156257
http://dx.doi.org/10.1186/s12864-020-6634-9