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CATS: Cas9-assisted tag switching. A high-throughput method for exchanging genomic peptide tags in yeast
BACKGROUND: The creation of arrays of yeast strains each encoding a different protein with constant tags is a powerful method for understanding how genes and their proteins control cell function. As genetic tools become more sophisticated there is a need to create custom libraries encoding proteins...
Autores principales: | Berry, Lisa K., Thomas, Grace Heredge, Thorpe, Peter H. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7063721/ https://www.ncbi.nlm.nih.gov/pubmed/32156257 http://dx.doi.org/10.1186/s12864-020-6634-9 |
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