Reversed paired-gRNA plasmid cloning strategy for efficient genome editing in Escherichia coli

BACKGROUND: Co-expression of two distinct guide RNAs (gRNAs) has been used to facilitate the application of CRISPR/Cas9 system in fields such as large genomic deletion. The paired gRNAs are often placed adjacently in the same direction and expressed individually by two identical promoters, constitut...

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Detalles Bibliográficos
Autores principales: Ding, Tingting, Huang, Chaoyong, Liang, Zeyu, Ma, Xiaoyan, Wang, Ning, Huo, Yi-Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7063769/
https://www.ncbi.nlm.nih.gov/pubmed/32156270
http://dx.doi.org/10.1186/s12934-020-01321-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7063769/
https://www.ncbi.nlm.nih.gov/pubmed/32156270
http://dx.doi.org/10.1186/s12934-020-01321-4

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