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Reversed paired-gRNA plasmid cloning strategy for efficient genome editing in Escherichia coli
BACKGROUND: Co-expression of two distinct guide RNAs (gRNAs) has been used to facilitate the application of CRISPR/Cas9 system in fields such as large genomic deletion. The paired gRNAs are often placed adjacently in the same direction and expressed individually by two identical promoters, constitut...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7063769/ https://www.ncbi.nlm.nih.gov/pubmed/32156270 http://dx.doi.org/10.1186/s12934-020-01321-4 |