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Predominant cleavage of proteins N-terminal to serines and threonines using scandium(III) triflate

ABSTRACT: Proteolytic digestion prior to LC–MS analysis is a key step for the identification of proteins. Digestion of proteins is typically performed with trypsin, but certain proteins or important protein sequence regions might be missed using this endoproteinase. Only few alternative endoproteina...

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Detalles Bibliográficos
Autores principales: Koehler, Christian J., Thiede, Bernd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7064626/
https://www.ncbi.nlm.nih.gov/pubmed/31667593
http://dx.doi.org/10.1007/s00775-019-01733-7