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Predominant cleavage of proteins N-terminal to serines and threonines using scandium(III) triflate
ABSTRACT: Proteolytic digestion prior to LC–MS analysis is a key step for the identification of proteins. Digestion of proteins is typically performed with trypsin, but certain proteins or important protein sequence regions might be missed using this endoproteinase. Only few alternative endoproteina...
Autores principales: | Koehler, Christian J., Thiede, Bernd |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7064626/ https://www.ncbi.nlm.nih.gov/pubmed/31667593 http://dx.doi.org/10.1007/s00775-019-01733-7 |
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