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Construction of E. coli—Mycobacterium shuttle vectors with a variety of expression systems and polypeptide tags for gene expression in mycobacteria

Cloning and expression of a desired gene is indispensable in molecular biology studies. Expression vectors, in this regard, should offer much needed flexibility and choice of cloning strategies for both in vivo and in vitro protein expression experiments. Furthermore, availability of option to choos...

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Detalles Bibliográficos
Autores principales: Seniya, Surya Pratap, Yadav, Priya, Jain, Vikas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7065818/
https://www.ncbi.nlm.nih.gov/pubmed/32160243
http://dx.doi.org/10.1371/journal.pone.0230282