High-Efficiency Multi-site Genomic Editing of Pseudomonas putida through Thermoinducible ssDNA Recombineering

Application of single-stranded DNA recombineering for genome editing of species other than enterobacteria is limited by the efficiency of the recombinase and the action of endogenous mismatch repair (MMR) systems. In this work we have set up a genetic system for entering multiple changes in the chro...

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Detalles Bibliográficos
Autores principales: Aparicio, Tomas, Nyerges, Akos, Martínez-García, Esteban, de Lorenzo, Víctor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7068128/
https://www.ncbi.nlm.nih.gov/pubmed/32179472
http://dx.doi.org/10.1016/j.isci.2020.100946

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7068128/
https://www.ncbi.nlm.nih.gov/pubmed/32179472
http://dx.doi.org/10.1016/j.isci.2020.100946

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