Programmable adenine deamination in bacteria using a Cas9–adenine-deaminase fusion

Precise genetic manipulation is vital to studying bacterial physiology, but is difficult to achieve in some bacterial species due to the weak intrinsic homologous recombination (HR) capacity and lack of a compatible exogenous HR system. Here we report the establishment of a rapid and efficient metho...

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Detalles Bibliográficos
Autores principales: Zhang, Ya, Zhang, Hongyuan, Wang, Zhipeng, Wu, Zhaowei, Wang, Yu, Tang, Na, Xu, Xuexia, Zhao, Suwen, Chen, Weizhong, Ji, Quanjiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2020
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7069399/
https://www.ncbi.nlm.nih.gov/pubmed/32206285
http://dx.doi.org/10.1039/c9sc03784e

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7069399/
https://www.ncbi.nlm.nih.gov/pubmed/32206285
http://dx.doi.org/10.1039/c9sc03784e

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