Cargando…
A novel family of expression vectors with multiple affinity tags for wheat germ cell-free protein expression
BACKGROUND: Cell-free protein expression has become a widely used alternative of in vivo, cell-based systems in functional and structural studies of proteins. The wheat germ-based method outstands from the commercially available eukaryotic in vitro translation systems by its flexibility, high transl...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7071761/ https://www.ncbi.nlm.nih.gov/pubmed/32169064 http://dx.doi.org/10.1186/s12896-020-00610-5 |