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Local rewiring of genome–nuclear lamina interactions by transcription
Transcriptionally inactive genes are often positioned at the nuclear lamina (NL), as part of large lamina‐associated domains (LADs). Activation of such genes is often accompanied by repositioning toward the nuclear interior. How this process works and how it impacts flanking chromosomal regions are...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073462/ https://www.ncbi.nlm.nih.gov/pubmed/32080885 http://dx.doi.org/10.15252/embj.2019103159 |
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author | Brueckner, Laura Zhao, Peiyao A van Schaik, Tom Leemans, Christ Sima, Jiao Peric‐Hupkes, Daniel Gilbert, David M van Steensel, Bas |
author_facet | Brueckner, Laura Zhao, Peiyao A van Schaik, Tom Leemans, Christ Sima, Jiao Peric‐Hupkes, Daniel Gilbert, David M van Steensel, Bas |
author_sort | Brueckner, Laura |
collection | PubMed |
description | Transcriptionally inactive genes are often positioned at the nuclear lamina (NL), as part of large lamina‐associated domains (LADs). Activation of such genes is often accompanied by repositioning toward the nuclear interior. How this process works and how it impacts flanking chromosomal regions are poorly understood. We addressed these questions by systematic activation or inactivation of individual genes, followed by detailed genome‐wide analysis of NL interactions, replication timing, and transcription patterns. Gene activation inside LADs typically causes NL detachment of the entire transcription unit, but rarely more than 50–100 kb of flanking DNA, even when multiple neighboring genes are activated. The degree of detachment depends on the expression level and the length of the activated gene. Loss of NL interactions coincides with a switch from late to early replication timing, but the latter can involve longer stretches of DNA. Inactivation of active genes can lead to increased NL contacts. These extensive datasets are a resource for the analysis of LAD rewiring by transcription and reveal a remarkable flexibility of interphase chromosomes. |
format | Online Article Text |
id | pubmed-7073462 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70734622020-03-18 Local rewiring of genome–nuclear lamina interactions by transcription Brueckner, Laura Zhao, Peiyao A van Schaik, Tom Leemans, Christ Sima, Jiao Peric‐Hupkes, Daniel Gilbert, David M van Steensel, Bas EMBO J Articles Transcriptionally inactive genes are often positioned at the nuclear lamina (NL), as part of large lamina‐associated domains (LADs). Activation of such genes is often accompanied by repositioning toward the nuclear interior. How this process works and how it impacts flanking chromosomal regions are poorly understood. We addressed these questions by systematic activation or inactivation of individual genes, followed by detailed genome‐wide analysis of NL interactions, replication timing, and transcription patterns. Gene activation inside LADs typically causes NL detachment of the entire transcription unit, but rarely more than 50–100 kb of flanking DNA, even when multiple neighboring genes are activated. The degree of detachment depends on the expression level and the length of the activated gene. Loss of NL interactions coincides with a switch from late to early replication timing, but the latter can involve longer stretches of DNA. Inactivation of active genes can lead to increased NL contacts. These extensive datasets are a resource for the analysis of LAD rewiring by transcription and reveal a remarkable flexibility of interphase chromosomes. John Wiley and Sons Inc. 2020-02-21 2020-03-16 /pmc/articles/PMC7073462/ /pubmed/32080885 http://dx.doi.org/10.15252/embj.2019103159 Text en © 2020 The Authors. Published under the terms of the CC BY NC ND 4.0 license This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Articles Brueckner, Laura Zhao, Peiyao A van Schaik, Tom Leemans, Christ Sima, Jiao Peric‐Hupkes, Daniel Gilbert, David M van Steensel, Bas Local rewiring of genome–nuclear lamina interactions by transcription |
title | Local rewiring of genome–nuclear lamina interactions by transcription |
title_full | Local rewiring of genome–nuclear lamina interactions by transcription |
title_fullStr | Local rewiring of genome–nuclear lamina interactions by transcription |
title_full_unstemmed | Local rewiring of genome–nuclear lamina interactions by transcription |
title_short | Local rewiring of genome–nuclear lamina interactions by transcription |
title_sort | local rewiring of genome–nuclear lamina interactions by transcription |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073462/ https://www.ncbi.nlm.nih.gov/pubmed/32080885 http://dx.doi.org/10.15252/embj.2019103159 |
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